By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Nanoparticles are presented in a new report. According to news reporting originating from Gainesville, Florida, by NewsRx correspondents, research stated, "Accurate mass analysis can provide useful information on the stoichiometry and composition of protein-based particles, such as virus-like assemblies. For applications in nanotechnology and medicine, such nanoparticles are loaded with foreign cargos, making accurate mass information essential to define the cargo load."
Our news editors obtained a quote from the research from the University of Florida, "Here, we describe modifications to an Orbitrap mass spectrometer that enable high mass analysis of several virus-like nanoparticles up to 4.5 MDa in mass. This allows the accurate determination of the composition of virus-like particles. The modified instrument is utilized to determine the cargo load of bacterial encapsulin nanoparticles that were engineered to encapsulate foreign cargo proteins. We find that encapsulin packages from 8 up to 12 cargo proteins, thereby quantifying cargo load but also showing the ensemble spread. In addition, we determined the previously unknown stoichiometry of the three different splice variants of the capsid protein in adeno-associated virus (AAV) capsids, showing that symmetry is broken and assembly is heterogeneous and stochastic."
According to the news editors, the research concluded: "These results demonstrate the potential of high-resolution mass analysis of protein-based nanoparticles, with widespread applications in chemical biology and nanotechnology."
For more information on this research see: Defining the Stoichiometry and Cargo Load of Viral and Bacterial Nanoparticles by Orbitrap Mass Spectrometry. Journal of the American Chemical Society, 2014;136(20):7295-7299. Journal of the American Chemical Society can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of the American Chemical Society - www.pubs.acs.org/journal/jacsat)
The news editors report that additional information may be obtained by contacting J. Snijder, University of Florida, Dept. of Biochem & Mol Biol, Struct Biol Center, McKnight Brain InstColl Med, Gainesville, FL 32610, United States. Additional authors for this research include M. van de Waterbeemd, E. Damoc, E. Denisov, D. Grinfeld, A. Bennett, M. Agbandje-McKenna, A. Makarov and A.J.R. Heck (see also Nanoparticles).
Keywords for this news article include: Florida, Gainesville, United States, Nanotechnology, Emerging Technologies, North and Central America
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