By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on DNA Research are discussed in a new report. According to news originating from Minneapolis, Minnesota, by NewsRx correspondents, research stated, "Benzene oxide, the initial metabolite of the human carcinogen benzene, reacts with DNA producing 7-phenylguanine (7-PhG) and other products. We developed a highly sensitive liquid chromatography-nanoelectrospray ionization-high resolution tandem mass spectrometry-parallel reaction monitoring method for the analysis of 7-PhG in DNA."
Our news journalists obtained a quote from the research from the University of Minnesota, "Accuracy and precision of the method were established and the detection limit was about 8 amol of 7-PhG injected on the column and less than 1 adduct per 109 nucleotides in DNA. 7-PhG was detected in calf thymus DNA reacted with 1 mu M to 10 mM benzene oxide. The method was applied for the analysis of DNA isolated from bone marrow, lung, and liver of B6C3F(1) mice treated by gavage with 50 mg/kg benzene in corn oil 5 times weekly for 4 weeks. 7-PhG was not detected in any of these DNA samples. The method was applied to DNA from mouse hepatocytes exposed to 100 mu M benzene oxide and human TK-6 lymphoblasts exposed to 100 mu M, 1, and 10 mM benzene oxide. 7-PhG was only detected in TK-6 cell DNA from the 10 mM exposure. The method was also applied to leukocyte DNA from 10 smokers and 10 nonsmokers. 7-PhG was detected in only one DNA sample, from a nonsmoker."
According to the news editors, the research concluded: "The results of this study do not support the hypothesis that the benzene oxide-DNA adduct 7-PhG is involved in carcinogenesis by benzene."
For more information on this research see: Analysis of the benzene oxide-DNA adduct 7-phenylguanine by liquid chromatography-nanoelectrospray ionization-high resolution tandem mass spectrometry-parallel reaction monitoring: Application to DNA from exposed mice and humans. Chemico-Biological Interactions, 2014;215():40-45. Chemico-Biological Interactions can be contacted at: Elsevier Ireland Ltd, Elsevier House, Brookvale Plaza, East Park Shannon, Co, Clare, 00000, Ireland. (Elsevier - www.elsevier.com; Chemico-Biological Interactions - www.elsevier.com/wps/product/cws_home/505510)
The news correspondents report that additional information may be obtained from A.T. Zarth, University of Minnesota, Masonic Canc Center, Minneapolis, MN 55455, United States. Additional authors for this research include G. Cheng, Z.B. Zhang, M.Y. Wang, P.W. Villalta, S. Balbo and S.S. Hecht (see also DNA Research).
Keywords for this news article include: Minnesota, Minneapolis, DNA Research, United States, North and Central America
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