News Column

Recent Findings from Seoul National University Has Provided New Information about Gene Therapy

June 26, 2014



By a News Reporter-Staff News Editor at Gene Therapy Weekly -- New research on Biotechnology is the subject of a report. According to news reporting originating from Seoul, South Korea, by NewsRx correspondents, research stated, "In this study, we characterized the polyamidoamine (PAMAM) dendrimer derivatives conjugated with basic amino acids as effective nonviral vector systems for gene delivery. Using PAMAM dendrimer (generation 4) as a core polymer, we further synthesized PAMAM G4-Histidine-Lysine (PAMAM G4-H-K) and PAMAM G4-Histidine-Ornithine (PAMAM G4-H-O)."

Our news editors obtained a quote from the research from Seoul National University, "Lysine and ornithine have cationic charged groups that can contribute to the condensation of DNA and interaction with cellular membranes. Histidine has an imidazole ring group that can induce a proton buffering effect. In this report, we performed experiment to evaluate the basic amino acid-PAMAM conjugates as efficient and safe gene carriers. The mean diameter and zeta potential value of the PAMAM conjugates/DNA complex were measured to be around 100 nm and 30 mV, respectively. It was observed that the PAMAM derivatives and plasmid DNA can form polyplexes at weight ratio 1.5 by agarose gel retardation and PicoGreen reagent assay. Furthermore, the PAMAM derivatives have shown high buffering capacity compared to the native PAMAM dendrimer. We performed the cytotoxicity and transfection assay in the HeLa, HepG2, HEK 293, and NIH3T3 cell lines. While the transfection efficiency was remarkable in all cell lines tested, the cytotoxicity level was very low."

According to the news editors, the research concluded: "Based on these characteristics, it is suggested that the basic amino acid-conjugated PAMAM dendrimers could be utilized as promising gene delivery polymeric vectors for effective gene therapy."

For more information on this research see: Basic amino acid-conjugated polyamidoamine dendrimers with enhanced gene transfection efficiency. Macromolecular Research, 2014;22(5):500-508. Macromolecular Research can be contacted at: Polymer Soc Korea, Room 601, Hatchon Building, 831 Yeoksam-Dong, Kangnam-Ku, Seoul 135-792, South Korea. (Springer - www.springer.com; Macromolecular Research - www.springerlink.com/content/1598-5032/)

The news editors report that additional information may be obtained by contacting J.H. Park, Seoul National University, Sch Chem & Mol Engn, Seoul 151742, South Korea. Additional authors for this research include J.S. Park and J.S. Choi (see also Biotechnology).

Keywords for this news article include: Asia, Biotechnology, Seoul, Peptides, Proteins, Histidine, South Korea, Gene Therapy, Bioengineering, Basic Amino Acids, Cyclic Amino Acids, Essential Amino Acids

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Source: Gene Therapy Weekly