By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Nanotechnology. According to news reporting originating in Villigen, Switzerland, by NewsRx journalists, research stated, "Simultaneous high-resolution imaging and localization of chemical interaction sites on single native proteins is a pertinent biophysical, biochemical, and nano-technological challenge. Such structural mapping and characterization of binding sites is of importance in understanding how proteins interact with their environment and in manipulating such interactions in a plethora of biotechnological applications."
The news reporters obtained a quote from the research from Paul Scherrer Institute, "Thus far, this challenge remains to be tackled. Here, we introduce force-distance curve-based atomic force microscopy (FD-based AFM) for the high-resolution imaging of SAS-6, a protein that self-assembles into cartwheel-like structures. Using functionalized AFM tips bearing Ni2+-N-nitrilotriacetate groups, we locate specific interaction sites on SAS-6 at nanometer resolution and quantify the binding strength of the Ni2+-NTA groups to histidine residues. The FD-based AFM approach can readily be applied to image any other native protein and to locate and structurally map histidine residues."
According to the news reporters, the research concluded: "Moreover, the surface chemistry used to functionalize the AFM tip can be modified to map other chemical interaction sites."
For more information on this research see: Localizing Chemical Groups while Imaging Single Native Proteins by High-Resolution Atomic Force Microscopy. Nano Letters, 2014;14(5):2957-2964. Nano Letters can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Nano Letters - www.pubs.acs.org/journal/nalefd)
Our news correspondents report that additional information may be obtained by contacting M. Pfreundschuh, Paul Scherrer Inst, Lab Biomol Res, Dept. of Biol & Chem, CH-5232 Villigen, Switzerland. Additional authors for this research include D. Alsteens, M. Hilbert, M.O. Steinmetz and D.J. Muller (see also Nanotechnology).
Keywords for this news article include: Europe, Villigen, Switzerland, Nanotechnology
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