News Column

New Findings from Central South University in Peptides and Proteins Provides New Insights

June 24, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Proteins are discussed in a new report. According to news reporting out of Changsha, People's Republic of China, by NewsRx editors, research stated, "Ultrasensitive detection of protein biomarkers is essential for early diagnosis and therapy of many diseases. Glycoproteins, differing from other types of proteins, contain carbohydrate moieties in the oligosaccharide chains."

Our news journalists obtained a quote from the research from Central South University, "Boronic acid can form boronate ester covalent bonds with diol-containing species. Herein, we present a sensitive and cost-effective electrochemical method for glycoprotein detection using 4-mercaptophenylboronic acid (MBA)/biotin-modified gold nanoparticles (AuNPs) (MBA-biotin-AuNPs) as labels. To demonstrate the feasibility and sensitivity of this method, recombinant human erythropoietin (rHuEPO) was tested as a model analyte. Specifically, rHuEPO was captured by the anti-rHuEPO aptamer-covered electrode and then derivatized with MBA-biotin-AuNPs through the boronic acid-carbohydrate interaction. The MBA-biotin-AuNPs facilitated the attachment of streptavidin-conjugated alkaline phosphatase for the production of electroactive p-aminophenol from p-aminophenyl phosphate substrate. A detection limit of 8 fmol L-1 for rHuEPO detection was achieved."

According to the news editors, the research concluded: "Other glycosylated and non-glycosylated proteins, such as horseradish peroxidase, prostate specific antigen, metallothionein, streptavidin, and thrombin showed no interference in the detection assay."

For more information on this research see: Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels. International Journal of Nanomedicine, 2014;9():2619-2626. International Journal of Nanomedicine can be contacted at: Dove Medical Press Ltd, PO Box 300-008, Albany, Auckland 0752, New Zealand (see also Proteins).

Our news journalists report that additional information may be obtained by contacting L. Liu, Central South University, Coll Chem & Chem Engn, Changsha, Hunan, People's Republic of China. Additional authors for this research include Y. Xing, H. Zhang, R.L. Liu, H.J. Liu and N. Xia.

Keywords for this news article include: Asia, Changsha, Nanoparticle, Glycoproteins, Nanotechnology, Glycoconjugates, Emerging Technologies, People's Republic of China

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Source: Life Science Weekly