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New Escherichia Coli Findings Has Been Reported by Investigators at Cornell University

June 24, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Proteobacteria. According to news reporting from Ithaca, New York, by NewsRx journalists, research stated, "The use of electrospun nanofibers as functional material in paper-based lateral flow assays (LFAs) was studied. Specific chemical features of the nanofibers were achieved by doping the base polymer, poly(lactic acid) (PLA), with poly(ethylene glycol) (PEG) and polystyrene(8K)-block-poly(ethylene-ran-butylene)(25K)-block-polyisoprene(10K)-Brij76 (K3-Brij76) (KB)."

The news correspondents obtained a quote from the research from Cornell University, "The LFAs were assembled such that the sample flowed through the nanofiber mat via capillary action. Initial investigations focused on the sustainable spinning and assembly of different polymer structures to allow the LFA format. Here, it was found that the base polymer poly(vinyl alcohol) (PVA), which was shown to function well in microfluidic biosensors, did not work in the LFA format. In contrast, PLA-based nanofibers enabled easy assembly. Three relevant features were chosen to study nanofiber-based functionalities in the LFA format: adsorption of antibodies, quantification of results, and nonspecific binding. In particular, streptavidin-conjugated sulforhodamine B (SRB)-encapsulating liposomes were captured by anti-streptavidin antibodies adsorbed on the nanofibers. Varying the functional polymer concentration within the PLA base enabled the creation of distinct capture zones. Also, a sandwich assay for the detection of Escherichia coli O157:H7 was developed using anti-E. coli antibodies as capture and reporter species with horseradish peroxidase for signal generation. A dose-response curve for E. coli with a detection limit of 1.9 x 10(4) cells was achieved. Finally, functional polymers were used to demonstrate that nonspecific binding could be eliminated using antifouling block copolymers."

According to the news reporters, the research concluded: "The enhancement of paper-based devices using functionalized nanofibers provides the opportunity to develop a broad spectrum of sensitive and specific bioassays with significant advantages over their traditional counterparts."

For more information on this research see: Developing new materials for paper-based diagnostics using electrospun nanofibers. Analytical and Bioanalytical Chemistry, 2014;406(14):3297-3304. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)

Our news journalists report that additional information may be obtained by contacting S.J. Reinholt, Cornell University, Dept. of Fiber Sci & Apparel Design, Ithaca, NY, United States. Additional authors for this research include A. Sonnenfeldt, A. Naik, M.W. Frey and A.J. Baeumner (see also Proteobacteria).

Keywords for this news article include: Antibodies, Ithaca, New York, Nanofiber, Immunology, Escherichia, United States, Blood Proteins, Nanotechnology, Immunoglobulins, Enterobacteriaceae, Gammaproteobacteria, Emerging Technologies, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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