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Investigators at Banaras Hindu University Report Findings in Enzymes and Coenzymes

June 24, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Enzymes and Coenzymes. According to news reporting out of Uttar Pradesh, India, by NewsRx editors, research stated, "Acid phosphatase (EC 3.1.3.2) from Vigna aconitifolia seeds was purified to apparent homogeneity by using ammonium sulfate fractionation and cation-exchange chromatography [carboxymethyl (CM) cellulose]. The enzyme was 228-fold purified with 14.6% recovery."

Our news journalists obtained a quote from the research from Banaras Hindu University, "Analytical gel filtration chromatography on Sephadex G-200 column showed that M-r of native enzyme was 58kDa and denaturing PAGE demonstrated that it was made up of two subunits of 24 and 27kDa. The enzyme showed its optimum activity at pH 5.0 and 60 degrees C. It exhibited broad substrate specificity and showed a higher specificity constant for para-nitrophenyl phosphate, Na -naphthyl phosphate, and adenosine monophosphate (AMP). Cu2+, Mo6+, Fe3+, phosphate, and fluoride ions were reported as strong inhibitors for the enzyme."

According to the news editors, the research concluded: "Active site study for the enzyme demonstrated that tryptophan and aspartic acid may be important for the catalysis."

For more information on this research see: Isolation and enzymatic properties of a nonspecific acid phosphatase from Vigna aconitifolia seeds. Biotechnology and Applied Biochemistry, 2014;61(2):145-152. Biotechnology and Applied Biochemistry can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Biotechnology and Applied Biochemistry - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1470-8744)

Our news journalists report that additional information may be obtained by contacting A. Anand, Banaras Hindu Univ, Fac Sci, Dept. of Biochem, Varanasi 221005, Uttar Pradesh, India (see also Enzymes and Coenzymes).

Keywords for this news article include: Asia, India, Uttar Pradesh, Enzymes and Coenzymes

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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