By a News Reporter-Staff News Editor at Agriculture Week -- New research on Integrative Agriculture is the subject of a report. According to news reporting originating from Lanzhou, People's Republic of China, by VerticalNews correspondents, research stated, "Copper nanoparticle is a new material widely used in biological medicine, animal husbandry and industrial areas, but its potential toxicity to human health and environment remains unclear. In order to study the hepatotoxic mechanisms of nanoparticles copper, two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI/TOF MS) of proteomics technology were used to isolate and identify the differentially expressed proteins from liver, which associated with hepatotoxicity induced by copper nanoparticle in rats."
Our news editors obtained a quote from the research from Gansu Agricultural University, "In this study, we have screened 15 kinds of proteins related with hepatotoxicity, of which spot8212 was identified as Malate dehydrogenase (Mdhl). The mRNA expression trend of Mdhl was consistent with the result of 2-DE by RT-PCR validation. Bioinformatics analysis showed that Mdhl was stable and no signal peptides, subcellular location was in endoplasmic reticulum; it contained many functional sites such as malate dehydrogenase activity signal sites 155LTRLDHNRAKSQI167; a helixes and random coils were the two main elements. Homologous analysis demonstrated high homologous of Mdh1 in rats with mouse and human, and the phylogenetic tree of Mdh1 was constructed. The result indicated that copper nanoparticle could regulate up the Mdhl protein expression so as to compensate the energy deficit."
According to the news editors, the research concluded: "Energy metabolic disturbance may be a pathway for copper nanoparticle particles to exert the hepatotoxic effects in rats."
For more information on this research see: Comparative Proteomic Analysis Shows an Elevation of Mdh1 Associated with Hepatotoxicity Induced by Copper Nanoparticle in Rats. Journal of Integrative Agriculture, 2014;13(5):1073-1081. Journal of Integrative Agriculture can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Journal of Integrative Agriculture - www.elsevier.com/wps/product/cws_home/727934)
The news editors report that additional information may be obtained by contacting S.W. Dong, Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, People's Republic of China. Additional authors for this research include Z.H. Gao, X.Y. Shen, H.W. Xue and X. Li.
Keywords for this news article include: Asia, Lanzhou, Dehydrogenase, Enzymes and Coenzymes, Integrative Agriculture, People's Republic of China
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