Project reference: 331285 Duration: From 2014-06-01 to 2016-05-31 Status: Active Phase : Accepted Total cost: EUR 161 968 (219249.60 US Dollar) EU contribution: EUR 161 968 (219249.60 US Dollar) Contract type: Intra-European Fellowships (IEF) Programme acronym: FP7-PEOPLE Subprogramme area: FP7-PEOPLE-2012-IEF Objective: Autophagy is a bulk cellular degradation pathway in which double-membrane vesicles engulfe large and long-lived cytoplasmic structures and target them to lysosomal compartments. Employing an integrated structural biology approach using single-particle cryo-EM and X-ray crystallography, this proposal aims to elucidate the structural details by which the autophagosomal recognition machinery identifies and assembles its cargo for targeting to autophagosomes. It has recently become apparent that selective autophagy requires specific receptors that recognize and recruit defined cargo into molecular assemblies and target them for autophagosomal degradation. Dysfunction of this process is implicated in a number of important human pathologies, such as cancer, neurodegeneration and myopathies. A precise understanding of the molecular mechanisms underlying selective autophagy requires the structural characterization of the molecular complexes involved in this process. First, the ultrastructural architecture of homo-oligomeric receptor assemblies will be established. Second, the structural organization of cargo-receptor complexes will be defined for a hetero-oligomeric, mechanosensitive model complex involved in muscle homeostasis using a combination of cryo-EM and X-ray crystallography. Third, oligomeric changes of cargo-receptor assemblies will be related to cellular function. In its aim to establish a multi-resolution view on the molecular principles of selective autophagy, this proposal has the potential to fundamentally advance our current understanding of this important degradation pathway.
Project completion date : 2016-05-31 12:00:00
Major organization : EUROPEAN MOLECULAR BIOLOGY LABORATORY
Address : Meyerhofstrasse 1, HEIDELBERG, DEUTSCHLAND Attn : Jillian ROWE (Ms.)
Url : http://www.embl.org/
Financier : European Union (EU)
Financier address : European Union (EU) Rue de la Loi 200/Wetstraat 200, B-1049 Bruxelles/Brussels, Belgium Tel: 32-2-2999696, 2993085 Fax: 32-2-2961749 Url: ec.europa.eu/