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Study Results from Whitehead Institute for Biomedical Research Broaden Understanding of Xylose Therapy

June 18, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Drugs and Therapies. According to news reporting out of Cambridge, Massachusetts, by NewsRx editors, research stated, "Phenotyping single cells based on the products they secrete or consume is a key bottleneck in many biotechnology applications, such as combinatorial metabolic engineering for the overproduction of secreted metabolites. Here we present a flexible high-throughput approach that uses microfluidics to compartmentalize individual cells for growth and analysis in monodisperse nanoliter aqueous droplets surrounded by an immiscible fluorinated oil phase."

Our news journalists obtained a quote from the research from Whitehead Institute for Biomedical Research, "We use this system to identify xylose-overconsuming Saccharomyces cerevisiae cells from a population containing one such cell per 10(4) cells and to screen a genomic library to identify multiple copies of the xylose isomerase gene as a genomic change contributing to high xylose consumption, a trait important for lignocellulosic feedstock utilization. We also enriched l-lactate-producing Escherichia coli clones 5,800x from a population containing one l-lactate producer per 104 d-lactate producers."

According to the news editors, the research concluded: "Our approach has broad applications for single-cell analyses, such as in strain selection for the overproduction of fuels, chemicals and pharmaceuticals."

For more information on this research see: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption. Nature Biotechnology, 2014;32(5):473-194. Nature Biotechnology can be contacted at: Nature Publishing Group, 75 Varick St, 9TH Flr, New York, NY 10013-1917, USA. (Nature Publishing Group - www.nature.com/; Nature Biotechnology - www.nature.com/nbt/)

Our news journalists report that additional information may be obtained by contacting B.L. Wang, Whitehead Inst Biomed Res, Cambridge, MA, United States. Additional authors for this research include A. Ghaderi, H. Zhou, J. Agresti, D.A. Weitz, G.R. Fink and G. Stephanopoulos (see also Drugs and Therapies).

Keywords for this news article include: Pharmaceuticals, Xylose, Cambridge, Massachusetts, United States, Drugs and Therapies, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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