News Column

Study Findings from Center for Nanoscience Provide New Insights into Molecular Biology

June 17, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Life Science Research is now available. According to news reporting from Munich, Germany, by NewsRx journalists, research stated, "Pulsed interleaved excitation (PIE) employs pulsed laser sources that are interleaved such that differentially colored fluorophores can be measured or imaged quasi-simultaneously in the absence of spectral crosstalk. PIE improves the robustness and reduces data analysis complexity of many fluorescence techniques, such as fluorescence cross-correlation spectroscopy (FCCS) and raster image cross-correlation spectroscopy (ccRICS), two methods used for quantitative investigation of molecular interactions in vitro and in living cells."

The news correspondents obtained a quote from the research from Center for Nanoscience, "However, as PIE is most often used for fluorescence fluctuation spectroscopy and burst analysis experiments and utilizes time-correlated single-photon counting detection and advanced optoelectronics, it has remained a technique that is mostly used by specialized single-molecule research groups. This protocols chapter provides an accessible overview of PIE for anyone considering implementing the method on a homebuilt or commercial microscope."

According to the news reporters, the research concluded: "We give details on the instrumentation, data collection and analysis software, on how to properly set up and align a PIE microscope, and finally, on how to perform proper dual-color FCS and RICS experiments."

For more information on this research see: Implementation and application of pulsed interleaved excitation for dual-color FCS and RICS. Methods In Molecular Biology, 2014;1076():653-82 (see also Life Science Research).

Our news journalists report that additional information may be obtained by contacting J. Hendrix, Physical Chemistry, Dept. of Chemistry, Munich Center for Integrated Protein Science (CiPSM) and Center for Nanoscience (CeNS), Ludwig-Maximilian-Universitat Munchen, Munich, Germany.

Keywords for this news article include: Munich, Europe, Germany, Life Science Research.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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