By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Nanomaterials have been published. According to news reporting from West Lafayette, Indiana, by NewsRx journalists, research stated, "There is an increasing need to develop biosensors for the detection of harmful pesticide residues in food and water. Here, we report on a versatile strategy to synthesize functionalized graphene oxide nanomaterials with abundant affinity groups that can capture histidine (His)-tagged acetylcholinesterase (AChE) for the fabrication of paraoxon biosensors."
The news correspondents obtained a quote from the research from Purdue University, "Initially, exfoliated graphene oxide (GO) was functionalized by a diazonium reaction to introduce abundant carboxyl groups. Then, N-alpha, N-alpha-bis (carboxymethyl)-L-lysine hydrate (NTA-NH2) and Ni2+ were anchored onto the GO based materials step by step. AChE was immobilized on the functionalized graphene oxide (FGO) through the specific binding between Ni-NTA and His-tag. A low anodic oxidation potential was observed due to an enhanced electrocatalytic activity and a large surface area brought about by the use of FGO. Furthermore, a sensitivity of 2.23 mu A mM (1) to the acetylthiocholine chloride (ATChCl) substrate was found for our composite covered electrodes. The electrodes also showed a wide linear response range from 10 mM to 1 mM (R-2 = 0.996), with an estimated detection limit of 3 mu M based on an S/N = 3. The stable chelation between Ni-NTA and His-tagged AChE endowed our electrodes with great short-term and long-term stability. In addition, a linear correlation was found between paraoxon concentration and the inhibition response of the electrodes to paraoxon, with a detection limit of 6.5 Chi 10 M-10."
According to the news reporters, the research concluded: "This versatile strategy provides a platform to fabricate graphene oxide based nanomaterials for biosensor applications."
For more information on this research see: Functionalized graphene oxide for the fabrication of paraoxon biosensors. Analytica Chimica Acta, 2014;827():86-94. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
Our news journalists report that additional information may be obtained by contacting H.Y. Zhang, Purdue University, Sch Mat Engn, West Lafayette, IN 47907, United States. Additional authors for this research include Z.F. Li, A. Snyder, J. Xie and L.A. Stanciu (see also Nanomaterials).
Keywords for this news article include: Indiana, Biosensing, United States, West Lafayette, Bioengineering, Bionanotechnology, Nanobiotechnology, Emerging Technologies, North and Central America
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