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Researchers from Texas A&M University Detail New Studies and Findings in the Area of Saccharomyces cerevisiae

June 17, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Saccharomyces cerevisiae. According to news reporting originating from College Station, Texas, by NewsRx correspondents, research stated, "Strains lacking and overexpressing the vacuolar iron (Fe) importer CCC1 were characterized using Mossbauer and EPR spectroscopies. Vacuolar Fe import is impeded in Delta ccc1 cells and enhanced in CCC1-up cells, causing vacuolar Fe in these strains to decline and accumulate, respectively, relative to WT cells."

Our news editors obtained a quote from the research from Texas A&M University, "Cytosolic Fe levels should behave oppositely. The Fe content of Delta ccc1 cells grown under low-Fe conditions was similar to that in WT cells. Most Fe was mitochondrial with some nonheme high spin (NHHS) Fe-II present. Delta ccc1 cells grown with increasing Fe concentration in the medium contained less total Fe, less vacuolar HS Fe-III, and more NHHS Fe-II than in comparable WT cells. As the Fe concentration in the growth medium increased, the concentration of HS Fe-III in Delta ccc1 cells increased to just 60% of WT levels, while NHHS Fe-II increased to twice WT levels, suggesting that the NHHS Fe-II was cytosolic. Delta ccc1 cells suffered more oxidative damage than WT cells, suggesting that the accumulated NHHS Fe-II promoted Fenton chemistry. The Fe concentration in CCC1-up cells was higher than in WT cells; the extra Fe was present as NHHS Fe-II and Fe-II and as Fe-III oxyhydroxide nanoparticles. These cells contained less mitochondrial Fe and exhibited less ROS damage than Delta ccc1 cells. CCC1-up cells were adenine-deficient on minimal medium; supplementing with adenine caused a decline of NHHS Fe-II suggesting that some of the NHHS Fell that accumulated in these cells was associated with adenine deficiency rather than the overexpression of CCC1. A mathematical model was developed that simulated changes in Fe distributions. Simulations suggested that only a modest proportion of the observed NHHS Fe-II in both strains was the cytosolic form of Fe that is sensed by the Fe import regulatory system."

According to the news editors, the research concluded: "The remainder is probably generated by the reduction of the vacuolar NHHS Fe-III species."

For more information on this research see: Mossbauer, EPR, and Modeling Study of Iron Trafficking and Regulation in Delta ccr1 and CCC1-up Saccharomyces cerevisiae. Biochemistry, 2014;53(18):2926-2940. Biochemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; Biochemistry -

The news editors report that additional information may be obtained by contacting A. Cockrell, Texas A&M University, Dept. of Chem, College Stn, TX 77843, United States. Additional authors for this research include S.P. McCormick, M.J. Moore, M. Chakrabarti and P.A. Lindahl (see also Saccharomyces cerevisiae).

Keywords for this news article include: Texas, United States, Life Sciences, College Station, Saccharomycetales, Saccharomycetaceae, Saccharomyces cerevisiae, North and Central America

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Source: Life Science Weekly

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