By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Immunology. According to news reporting out of Seattle, Washington, by NewsRx editors, research stated, "Colloidal stability of antibody solutions, i.e., the propensity of the folded protein to precipitate, is an important consideration in formulation development of therapeutic monoclonal antibodies. In a protein solution, different pathways including crystallization, colloidal aggregation, and liquid-liquid phase separation (LLPS) can lead to the formation of precipitates."
Our news journalists obtained a quote from the research from Amgen, "The kinetics of crystallization and aggregation are often slow and vary from protein to protein. Due to the diverse mechanisms of these protein condensation processes, it is a challenge to develop a standardized test for an early evaluation of the colloidal stability of antibody solutions. LLPS would normally occur in antibody solutions at sufficiently low temperature, provided that it is not preempted by freezing of the solution. Poly(ethylene glycol) (PEG) can be used to induce LLPS at temperatures above the freezing point. Here, we propose a colloidal stability test based on inducing LLPS in antibody solutions and measuring the antibody concentration of the dilute phase. We demonstrate experimentally that such a PEG-induced LLPS test can be used to compare colloidal stability of different antibodies in different solution conditions and can be readily applied to high-throughput screening. We have derived an equation for the effects of PEG concentration and molecular weight on the results of the LLPS test. Finally, this equation defines a binding energy in the condensed phase, which can be determined in the PEG-induced LLPS test."
According to the news editors, the research concluded: "This binding energy is a measure of attractive interactions between antibody molecules and can be used for quantitative characterization of the colloidal stability of antibody solutions."
For more information on this research see: Quantitative Evaluation of Colloidal Stability of Antibody Solutions using PEG-Induced Liquid-Liquid Phase Separation. Molecular Pharmaceutics, 2014;11(5):1391-1402. Molecular Pharmaceutics can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Molecular Pharmaceutics - www.pubs.acs.org/journal/mpohbp)
Our news journalists report that additional information may be obtained by contacting Y. Wang, Amgen Inc, Proc & Prod Dev, Seattle, WA 98119, United States. Additional authors for this research include R.F. Latypov, A. Lomakin, J.A. Meyer, B.A. Kerwin, S. Vunnum and G.B. Benedek (see also Immunology).
Keywords for this news article include: Antibodies, Seattle, Washington, Immunology, United States, Blood Proteins, Immunoglobulins, North and Central America
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