By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on DNA Research. According to news reporting out of Urbana, Illinois, by NewsRx editors, research stated, "Practical applications of solid-state nanopores for DNA detection and sequencing require the electrophoretic motion of DNA through the nanopores to be precisely controlled. Controlling the motion of single-stranded DNA presents a particular challenge, in part because of the multitude of conformations that a DNA strand can adopt in a nanopore."
Our news journalists obtained a quote from the research from the University of Illinois, "Through continuum, coarse-grained and atomistic modeling, we demonstrate that local heating of the nanopore volume can be used to alter the electrophoretic mobility and conformation of single-stranded DNA. In the nanopore systems considered, the temperature near the nanopore is modulated via a nanometer-size heater element that can be radiatively switched on and off. The local enhancement of temperature produces considerable stretching of the DNA fragment confined within the nanopore. Such stretching is reversible, so that the conformation of DNA can be toggled between compact (local heating is off) and extended (local heating is on) states. The effective thermophoretic force acting on single-stranded DNA in the vicinity of the nanopore is found to be sufficiently large (4-8 pN) to affect such changes in the DNA conformation."
According to the news editors, the research concluded: "The local heating of the nanopore volume is observed to promote single-file translocation of DNA strands at transmembrane biases as low as 10 mV, which opens new avenues for using solid-state nanopores for detection and sequencing of DNA."
For more information on this research see: Stretching and controlled motion of single-stranded DNA in locally heated solid-state nanopores. Acs Nano, 2013;7(8):6816-24. (American Chemical Society - www.acs.org; Acs Nano - www.pubs.acs.org/journal/ancac3)
Our news journalists report that additional information may be obtained by contacting M. Belkin, Dept. of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States. Additional authors for this research include C. Maffeo, D.B. Wells and A. Aksimentiev (see also DNA Research).
Keywords for this news article include: Urbana, Illinois, DNA Research, United States, North and Central America.
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