News Column

Reports from Prince of Songkla University Add New Data to Findings in Hela Cells

June 19, 2014



By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Current study results on Hela Cells have been published. According to news reporting originating in Songkhla, Thailand, by NewsRx journalists, research stated, "The aim of this study was to optimize transfection efficiency (TE) of the depolymerized low molecular weight (LW) chitosan with molecular weight (Mw) at 16 kDa and 54% degree of deacetylation (DDA) on three primary cells of fibroblast (F), dental pulp (P), and periodontal ligament (PDL). The effect of low frequency ultrasound treatment on the chitosan-DNA complexes prior transfection on TE was also evaluated."

The news reporters obtained a quote from the research from the Prince of Songkla University, "This LW chitosan required high N/P ratio (>34) to bind DNA completely. An N/P ratio above 56 tended to improve TE in most primary cells nearly at the level of Lipofectamine. Ultrasonication can reduce the aggregation and sizes of the chitosan-DNA microparticles. It increased TE of F cells at an N/P ratio above 34, which was higher than Lipofectamine. However, this ultrasound treatment caused loss of TE in KB cells. MTT assay of these chitosan-DNA complexes revealed no significant cytotoxicity to both KB and F cells."

According to the news reporters, the research concluded: "This LW chitosan has potential for further development into a safer alternative to gene delivery systems in various cells of interest; however the optimal conditions have to be adjusted, depending on each cell source."

For more information on this research see: Ultrasound treatment increases transfection efficiency of low molecular weight chitosan in fibroblasts but Not in KB cells. Plos One, 2014;9(3):e92076. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)

Our news correspondents report that additional information may be obtained by contacting U. Kedjarune-Leggat, Dept. of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Songkhla, Thailand. Additional authors for this research include C. Supaprutsakul and W. Chotigeat (see also Hela Cells).

Keywords for this news article include: Asia, Biotechnology, Songkhla, Thailand, KB Cells, Cell Line, Hela Cells, Fibroblasts, DNA Research, Gene Therapy, Bioengineering, Connective Tissue Cells.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Gene Therapy Weekly


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