By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Life Science Research are presented in a new report. According to news reporting originating in Karlsruhe, Germany, by NewsRx journalists, research stated, "E-cadherin is a key cell-cell adhesion molecule but the impact of receptor density and the precise contribution of individual cadherin ectodomains in promoting cell adhesion are only incompletely understood. Investigating these mechanisms would benefit from artificial adhesion substrates carrying different cadherin ectodomains at defined surface density."
The news reporters obtained a quote from the research from the Karlsruhe Institute of Technology, "We therefore developed a quantitative E-cadherin surface immobilization protocol based on the SNAP-tag technique. Extracellular (EC) fragments of E-cadherin fused to the SNAP-tag were covalently bound to self-assembled monolayers (SAM) of thiols carrying benzylguanine (BG) head groups. The adhesive functionality of the different E-cadherin surfaces was then assessed using cell spreading assays and single-cell (SCSF) and single-molecule (SMSF) force spectroscopy. We demonstrate that an E-cadherin construct containing only the first and second outmost EC domain (E1-2) is not sufficient for mediating cell adhesion and yields only low single cadherin-cadherin adhesion forces. In contrast, a construct containing all five EC domains (E1-5) efficiently promotes cell spreading and generates strong single cadherin and cell adhesion forces. By varying the concentration of BG head groups within the SAM we determined a lateral distance of 5-11 nm for optimal E-cadherin functionality. Integrating the results from SCMS and SMSF experiments furthermore demonstrated that the dissolution of E-cadherin adhesion contacts involves a sequential unbinding of individual cadherin receptors rather than the sudden rupture of larger cadherin receptor clusters."
According to the news reporters, the research concluded: "Our method of covalent, oriented and density-controlled E-cadherin immobilization thus provides a novel and versatile platform to study molecular mechanisms underlying cadherin-mediated cell adhesion under defined experimental conditions."
For more information on this research see: Covalent and density-controlled surface immobilization of E-cadherin for adhesion force spectroscopy. Plos One, 2014;9(3):e93123. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news correspondents report that additional information may be obtained by contacting D. Fichtner, Karlsruhe Institute of Technology (KIT), DFG-Center for Functional Nanostructures, Karlsruhe, Germany. Additional authors for this research include B. Lorenz, S. Engin, C. Deichmann, M. Oelkers, A. Janshoff, A. Menke, D. Wedlich and C.M Franz (see also Life Science Research).
Keywords for this news article include: Europe, Germany, Karlsruhe, Life Science Research.
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