By a News Reporter-Staff News Editor at Health & Medicine Week -- New research on Proteobacteria is the subject of a report. According to news reporting originating from Jiangsu, People's Republic of China, by NewsRx correspondents, research stated, "A simple colorimetric sensing method was developed for detection of invA gene of Salmonella by combining DNAzyme probe self-assembled gold nanoparticles and polymerase chain reaction (PCR) technique. Amino-modified capture probe was covalently immobilized on the surface of DNA-BIND plate, and further hybridized with target sequence and DNAzyme probe self-assembled gold nanoparticles to form a sandwich-type hybridization structure."
Our news editors obtained a quote from the research from Nanjing University, "In the presence of hemin, the DNAzyme probe could form a G-quadruplex/hemin complex, which catalyzed the H2O2-mediated oxidation of 3,3 ',5,5 '-tetramethylbenzidine dihydrochloride hydrate (TMB) to cause a dramatic color change. The invA gene was isolated from Salmonella and amplified using a polymerase chain reaction technique, and signal was further enhanced by DNAzyme probe self-assembled gold nanoparticles. Under optimal conditions, the absorbance value was linear with the logarithm of target sequence concentrations in the range of 0.5-50 nM, with a limit of detection of 0.44 nM for target sequence. In addition, the designed method allowed the quantitative Salmonella detection in water from 3 Chi 10(3) to 3 Chi 10(6) CFU mL(-1)."
According to the news editors, the research concluded: "This strategy exhibited good stability, sensitivity and simplicity and might be a potential tool for the practical Salmonella detection in clinical diagnosis, food safety and environmental monitoring."
For more information on this research see: A colorimetric assay method for invA gene of Salmonella using DNAzyme probe self-assembled gold nanoparticles as single tag. Sensors and Actuators B-Chemical, 2014;198():87-93. Sensors and Actuators B-Chemical can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also Proteobacteria).
The news editors report that additional information may be obtained by contacting R. Luo, Nanjing University, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, People's Republic of China. Additional authors for this research include Y.H. Li, X.J. Lin, F. Dong, W. Zhang, L. Yan, W. Cheng, H.X. Ju and S.J. Ding.
Keywords for this news article include: Asia, Jiangsu, Salmonellosis, Nanotechnology, Enterobacteriaceae, Gold Nanoparticles, Gammaproteobacteria, Emerging Technologies, Gram-Negative Bacteria, People's Republic of China, Gram-Negative Facultatively Anaerobic Rods
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