By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Reoviridae Infections. According to news originating from Cuernavaca, Mexico, by NewsRx correspondents, research stated, "Rotavirus VP6 nanotubes are an attractive option for a recombinant vaccine against rotavirus disease. Protection against rotavirus infection and an adjuvant effect have been observed upon immunization with VP6 nanotubes."
Our news journalists obtained a quote from the research from National Autonomous University, "However, little information exists on how VP6 nanotubes interact with cells and trigger an immune response. In this work, the interaction between VP6 nanotubes and different cell lines was characterized. VP6 nanotubes were not cytotoxic to any of the animal or human cell lines tested. Uptake of nanotubes into cells was cell-line-dependent, as only THP1 and J774 macrophage cells internalized them. Moreover, the size and spatial arrangement of VP6 assembled into nanotubes allowed their uptake by macrophages, as double-layered rotavirus-like particles also displaying VP6 in their surface were not taken up. The internalization of VP6 nanotubes was inhibited by methyl-beta-cyclodextrin, but not by genistein, indicating that nanotube entry is specific, depends on the presence of cholesterol in the plasma membrane, and does not require the activity of tyrosine kinases."
According to the news editors, the research concluded: "The information generated here expands our understanding of the interaction of protein nanotubes with cells, which is useful for the application of VP6 nanotubes as a vaccine."
For more information on this research see: Understanding internalization of rotavirus VP6 nanotubes by cells: towards a recombinant vaccine. Archives of Virology, 2014;159(5):1005-1015. Archives of Virology can be contacted at: Springer Wien, Sachsenplatz 4-6, PO Box 89, A-1201 Wien, Austria. (Springer - www.springer.com; Archives of Virology - www.springerlink.com/content/0304-8608/)
The news correspondents report that additional information may be obtained from M. Rodriguez, National Autonomous University of Mexico, Inst Biotecnol, Dept. of Biol Mol Plantas, Cuernavaca 62250, Mor, Mexico. Additional authors for this research include C. Wood, R. Sanchez-Lopez, R.M. Castro-Acosta, O.T. Ramirez and L.A. Palomares (see also Reoviridae Infections).
Keywords for this news article include: Mexico, Cuernavaca, RNA Viruses, RNA Virus Infections, Rotavirus Infections, Reoviridae Infections, North and Central America
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