News Column

New Molecular Biology Findings Reported from Technical University

June 17, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Life Science Research. According to news originating from Lisbon, Portugal, by NewsRx correspondents, research stated, "Fluorescence correlation spectroscopy (FCS) is a powerful method to investigate molecular interactions based on the variation of diffusion properties at the single-molecule level. This technique allows studying quantitatively the interaction of fluorescently labeled proteins/peptides with lipid vesicles."

Our news journalists obtained a quote from the research from Technical University, "Here, we describe how to acquire and analyze FCS partition data in order to accurately determine the protein/peptide partition coefficients between the aqueous and lipid phases. It is shown that the recovery of unbiased partition coefficients from FCS partition curves (fractional amplitude of the bound species versus lipid concentration) requires considering explicitly the Poissonian loading of the lipid vesicles with the fluorescently labeled protein in order to account for the variable liposome brightness in each sample."

According to the news editors, the research concluded: "Additionally, the impact of a trace amount of a fluorescent non-binding component on the partition curves determined by FCS is also discussed."

For more information on this research see: Quantifying lipid-protein interaction by fluorescence correlation spectroscopy (FCS). Methods In Molecular Biology, 2014;1076():575-95 (see also Life Science Research).

The news correspondents report that additional information may be obtained from A.M. Melo, Centro de Quimica-Fisica Molecular and Institute of Nanoscience and Nanotechnology, Instituto Superior Tecnico, Universidade Tecnica de Lisboa, Lisbon, Portugal. Additional authors for this research include M. Prieto and A. Coutinho.

Keywords for this news article include: Lisbon, Europe, Portugal, Life Science Research.

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Source: Life Science Weekly