News Column

New Findings in Antisense Technology Described from Osaka University

June 18, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Biotechnology have been published. According to news reporting originating from Osaka, Japan, by NewsRx correspondents, research stated, "Gene knockdowns using oligonucleotide-based approaches are useful for studying gene function in both in vitro cell culture systems and in vivo animal models. We evaluated the efficacy of 2 ',4 '-bridged nucleic acids (BNA)-modified antisense oligonucleotides (AONs) for gene knockdown in zebrafish."

Our news editors obtained a quote from the research from Osaka University, "We used the tcf7l1a gene as a model for testing the knockdown efficacy of 2 ',4 '-BNA AONs and examined how the target sites/affinity and RNase H induction activity of 2 ',4 '-BNA AONs affect knockdown efficacy. We found that tcf7l1a gene function was knocked down by 2 ',4 '-BNA AONs that target the start codon and induce RNase H activity. Although nonspecific p53-mediated developmental defects were observed at higher doses, the effective dose of the 2 ',4 '-BNA AONs for tcf7l1a is much lower than that of morpholino oligonucleotides."

According to the news editors, the research concluded: "Our data thus show a potential application for 2 ',4 '-BNA AONs in the downregulation of specific genes in zebrafish."

For more information on this research see: Target Gene Knockdown by 2 ',4 '-BNA/LNA Antisense Oligonucleotides in Zebrafish. Nucleic Acid Therapeutics, 2014;24(3):186-191. Nucleic Acid Therapeutics can be contacted at: Mary Ann Liebert, Inc, 140 Huguenot Street, 3RD Fl, New Rochelle, NY 10801, USA. (Mary Ann Liebert, Inc. -; Nucleic Acid Therapeutics -

The news editors report that additional information may be obtained by contacting M. Itoh, Osaka University, Grad Sch Pharmaceut Sci, Suita, Osaka, Japan. Additional authors for this research include M. Nakaura, T. Imanishi and S. Obika (see also Biotechnology).

Keywords for this news article include: Asia, Antisense Technology, Biotechnology, Osaka, Japan, Bioengineering

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Source: Biotech Week

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