By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Current study results on Nanomedicine have been published. According to news reporting out of Catanzaro, Italy, by NewsRx editors, research stated, "The aim of this study was the evaluation of the effects of two emulsifiers on the physicochemical and technological properties of low molecular weight chitosan/poly (D,L-lactide-co-glycolide) (PLGA) nanoplexes and their transfection efficiency. Nanospheres were prepared using the nanoprecipitation method of the preformed polymer."
Our news journalists obtained a quote from the research from the University of Catanzaro Magna Graecia, "The mean diameter and surface charge of the nanospheres were investigated by photocorrelation spectroscopy. The degree of binding of the plasmid with the nanoplexes was qualitatively and quantitatively determined. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) testing was performed using HeLa, RPMI8226, and SKMM1 cell lines. Flow cytometry and confocal laser scanning microscopy were used to determine the degree of cellular transfection and internalization of the nanoplexes into cells, respectively. The nanoplexes had a positive zeta potential, and low amounts of PLGA and poloxamer 188 showed a mean colloidal size of similar to 200 nm with a polydispersity index of similar to 0.14. The nanoplexes had suitable entrapment efficiency (80%). In vitro experiments showed that the colloidal nanodevices did not induce significant cytotoxicity. The nanoplexes investigated in this study could represent efficient and useful nonviral devices for gene delivery. Use of low amounts of PLGA and poloxamer 188 enabled development of a nanosphere able to transfect cells efficiently."
According to the news editors, the research concluded: "These nanosystems are a helpful platform for delivery of genetic material while preserving therapeutic efficacy."
For more information on this research see: Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes. International Journal of Nanomedicine, 2014;9():2359-2372. International Journal of Nanomedicine can be contacted at: Dove Medical Press Ltd, PO Box 300-008, Albany, Auckland 0752, New Zealand (see also Nanomedicine).
Our news journalists report that additional information may be obtained by contacting D. Cosco, Univ Catanzaro Magna Graecia, Interreg Res Center Food Safety & Hlth, Catanzaro, Italy. Additional authors for this research include C. Federico, J. Maiuolo, S. Bulotta, R. Molinaro, D. Paolino, P. Tassone and M. Fresta.
Keywords for this news article include: Biotechnology, Italy, Europe, Catanzaro, Gene Therapy, Nanomedicine, Bioengineering
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