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New Findings from National Institute of Advanced Industrial Science and Technology (NIAIST) Describe Advances in Enzymes and Coenzymes

June 18, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Enzymes and Coenzymes. According to news originating from Ibaraki, Japan, by NewsRx correspondents, research stated, "There is a need to speed up the degradation of used agricultural mulch films that are made of biodegradable plastics (BPs) in the field. Treating them with BP-degrading enzymes could be a solution to this problem."

Our news journalists obtained a quote from the research from the National Institute of Advanced Industrial Science and Technology (NIAIST), "A cutinase-like enzyme of yeast Pseudozyma antarctica (PaE) has wide specificity of BPs degradation, but needs to be produced efficiently. Here we report that the production of PaE by P. antarctica can be increased by using xylose as carbon source. BP-degradation activity was analyzed using a polybutylene succinate-co-adipate (PBSA) emulsion as the substrate. Strain P. antarctica GB-4(1)W was found to be the best PaE producer among the tested strains. Using a 5-L jar fermentor with xylose fed-batch cultivation, high PaE productivity could be maintained and about 21 U/ml of PaE was obtained in 120 h. This amount was 100 times higher than the amount that we obtained previously (0.21 U/ml by flask cultivation using glycerol as carbon source). Under repeated xylose fed-batch cultivation with 24 h intervals, the maximum PaE production rate (0.34 U/ml/h) was maintained and the highest PaE productivity (28,000 U/2 L/d) was repeatedly obtained for 7 intervals."

According to the news editors, the research concluded: "The activity of filtered jar-culture (crude PaE) was stable over 12 weeks at 4 degrees C. Commercially available BP mulch films (20 pm thickness, cut into 1-cm-squares) were completely degraded by submerging them in crude PaE (2 U/ml) at 30 degrees C in 24 h. These results indicated that concentrated PaE can rapidly degrade the strength of BP mulch films in the field so that they do not interfere with plowing."

For more information on this research see: Xylose induces the phyllosphere yeast Pseudozyma antarctica to produce a cutinase-like enzyme which efficiently degrades biodegradable plastics. Journal of Bioscience and Bioengineering, 2014;117(3):325-329. Journal of Bioscience and Bioengineering can be contacted at: Soc Bioscience Bioengineering Japan, Osaka Univ, Faculty Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. (Elsevier -; Journal of Bioscience and Bioengineering -

The news correspondents report that additional information may be obtained from T. Watanabe, Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058565, Japan. Additional authors for this research include Y. Shinozaki, S. Yoshida, M. Koitabashi, Y. Sameshima-Yamashita, T. Fujii, T. Fukuoka and H.K. Kitamoto (see also Enzymes and Coenzymes).

Keywords for this news article include: Asia, Japan, Ibaraki, Enzymes and Coenzymes

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Source: Biotech Week

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