News Column

New Findings from Department of Physiology Describe Advances in Nanoparticles

June 17, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Nanoparticles. According to news originating from Berlin, Germany, by NewsRx correspondents, research stated, "The aim of the study was to establish a user-friendly approach for single fluorescence particle 3D localization and tracking with nanometre precision in a standard fluorescence microscope using a point spread function (PSF) approach, and to evaluate validity and precision for different analysis methods and optical conditions with particular application to microcirculatory flow dynamics and cell biology. Images of fluorescent particles were obtained with a standard fluorescence microscope equipped with a piezo positioner for the objective."

Our news journalists obtained a quote from the research from the Department of Physiology, "Whole pattern (WP) comparison with a PSF recorded for the specific set-up and measurement of the outermost ring radius (ORR) were used for analysis. Images of fluorescent particles were recorded over a large range (about 7?m) of vertical positions, with and without distortion by overlapping particles as well as in the presence of cultured endothelial cells. For a vertical range of 6.5?m the standard deviation (SD) from the predicted value, indicating validity, was 9.3/8.7 nm (WP/ORR) in the vertical and 8.2/11.7 nm in the horizontal direction. The precision, determined by repeated measurements, was 5.1/3.8 nm in the vertical and 2.9/3.7 nm in the horizontal direction. WP was more robust with respect to underexposure or overlapping images. On the surface of cultured endothelial cells, a layer with 2.5 times increased viscosity and a thickness of about 0.8?m was detected."

According to the news editors, the research concluded: "With a validity in the range of 10 nm and a precision down to about 3-5 nm obtained by standard fluorescent microscopy, the PSF approach offers a valuable tool for a variety of experimental investigations of particle localizations, including the assessment of endothelial cell microenvironment."

For more information on this research see: Tracking of fluorescence nanoparticles with nanometre resolution in a biological system: assessing local viscosity and microrheology. Biomechanics and Modeling In Mechanobiology, 2014;13(2):275-88. (Springer - www.springer.com; Biomechanics and Modeling In Mechanobiology - www.springerlink.com/content/1617-7959/)

The news correspondents report that additional information may be obtained from A. Marki, Dept. of Physiology, Charite Universitatsmedizin, Chariteplatz 1, 10117 , Berlin, Germany. Additional authors for this research include E. Ermilov, A. Zakrzewicz, A. Koller, T.W. Secomb and A.R Pries (see also Nanoparticles).

Keywords for this news article include: Berlin, Europe, Germany, Nanotechnology, Emerging Technologies.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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