By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Cytoplasmic Structures. According to news reporting originating in Mol, Belgium, by NewsRx journalists, research stated, "The activated leukocyte cell adhesion molecule (ALCAM) is involved in immune responses and cancer metastasis. ALCAM is linked to the actin cytoskeleton through a supramolecular complex, including ezrin and syntenin-1."
The news reporters obtained a quote from the research from Flemish Institute for Technological Research, "ALCAM supramolecular complex engaged to CD6 stabilizes the immunological synapse. Insights into the immunological synapse at the dendritic cell side contribute to clarify immune regulation mechanisms. Activated leukocyte cell adhesion molecule (ALCAM) is a type I transmembrane protein member of the immunoglobulin superfamily of cell adhesion molecules. Involved in important pathophysiological processes such as the immune response, cancer metastasis, and neuronal development, ALCAM undergoes both homotypic interactions with other ALCAM molecules and heterotypic interactions with the surface receptor CD6 expressed at the T cell surface. Despite biochemical and biophysical evidence of a dynamic association between ALCAM and the actin cytoskeleton, no detailed information is available about how this association occurs at the molecular level. Here, we exploit a combination of complementary microscopy techniques, including FRET detected by fluorescence lifetime imaging microscopy and single-cell force spectroscopy, and we demonstrate the existence of a preformed ligand-independent supramolecular complex where ALCAM stably interacts with actin by binding to syntenin-1 and ezrin. Interaction with the ligand CD6 further enhances these multiple interactions."
According to the news reporters, the research concluded: "Altogether, our results propose a novel biophysical framework to understand the stabilizing role of the ALCAM supramolecular complex engaged to CD6 during dendritic cell-T cell interactions and provide novel information on the molecular players involved in the formation and signaling of the immunological synapse at the dendritic cell side."
For more information on this research see: Syntenin-1 and Ezrin Proteins Link Activated Leukocyte Cell Adhesion Molecule to the Actin Cytoskeleton. Journal of Biological Chemistry, 2014;289(19):13445-13460. Journal of Biological Chemistry can be contacted at: Amer Soc Biochemistry Molecular Biology Inc, 9650 Rockville Pike, Bethesda, MD 20814-3996, USA. (American Society for Biochemistry and Molecular Biology - www.asbmb.org; Journal of Biological Chemistry - www.jbc.org/)
Our news correspondents report that additional information may be obtained by contacting C. Tudor, Flemish Inst Technol Res, Environm Risk & Hlth Unit, B-2400 Mol, Belgium. Additional authors for this research include J. te Riet, C. Eich, R. Harkes, N. Smisdom, J.B. Wenger, M. Ameloot, M. Holt, J.S. Kanger, C.G. Figdor, A. Cambi and V. Subramaniam (see also Cytoplasmic Structures).
Keywords for this news article include: Antigen-Presenting Cells, Europe, Belgium, Peptides, Amino Acids, Blood Cells, CD Antigens, Cytoskeleton, Cell Membrane, Immune System, Nanotechnology, Supramolecular, Dendritic Cells, Surface Antigens, Membrane Proteins, Biological Factors, Cellular Structures, Intracellular Space, Emerging Technologies
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