By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Life Science Research are discussed in a new report. According to news reporting originating in Kochi, Japan, by NewsRx journalists, research stated, "We attempted to overexpress three types of expression cassettes, each of which contained a different open reading frame (ORF) of domestic Chlamydomonas cDNAs. Each ORF was strongly driven by an artificial hybrid promoter."
The news reporters obtained a quote from the research from the Kochi University of Technology, "We used two wild-type Chlamydomonas strains (i.e., CC-124 and CC-125) and two mutant strains [i.e., UV-mutated (UVM) 4 and UVM11] that have been reported to have a high potency for expressing nondomestic nuclear transgenes. We found that the 1-deoxy-d-xylulose-5-phosphatesynthase (DXS1), 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR1), and squalene synthase (SQS) cassettes were not readily overexpressed in the wild-type strains at levels where the products were clearly detectable by Western blotting using a monoclonal antibody. In contrast, Western blot-positive SQS cassette transformants were frequently detected in the UVM4 and UVM11 strains, i.e., at an approximately 4.5 times higher frequency than that in the CC-124 wild-type strain. Moreover, transformants that accumulated large amounts of the SQS protein were obtained frequently in the UVM4 and UVM11 strains, i.e., the frequency was approximately 2.2 times higher than that in the CC-124 strain. However, a position effect of the integrated expression cassette was obviously detected not only in the wild-type but also in UVM strains. This suggests that the epigenetic repression mechanism of transgenic genes was not completely knocked out, even in the UVM strains."
According to the news reporters, the research concluded: "Further improved Chlamydomonas strains are essential to facilitate high-throughput screening of transformants that express nuclear transgenes at a high level."
For more information on this research see: Expression levels of domestic cDNA cassettes integrated in the nuclear genomes of various Chlamydomonas reinhardtii strains. Journal of Bioscience and Bioengineering, 2014;117(5):613-6. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
Our news correspondents report that additional information may be obtained by contacting F. Kong, School of Environmental Science and Engineering, Kochi University of Technology (KUT), Tosayamada, Kochi 782-8502, Japan. Additional authors for this research include T. Yamasaki and T. Ohama (see also Life Science Research).
Keywords for this news article include: Asia, Kochi, Japan, Genetics, Life Science Research.
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