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New Adenovirus Findings Has Been Reported by Investigators at University Nova of Lisboa

June 17, 2014



By a News Reporter-Staff News Editor at Virus Weekly -- Researchers detail new data in Adenovirus. According to news originating from Caparica, Portugal, by NewsRx correspondents, research stated, "The impacts of quaternary amine ligand density and matrix structure, namely hydrogel grafted and directly grafted, on state-of-the-art chromatographic membranes operated in bind-and-elute mode were evaluated for the purification of adenovirus serotype 5. The experiments were performed on a 96-well plate membrane holder, which is a convenient high-throughput screening tool for obtaining the best operating conditions for a process yield optimization. The results show that the hydrogel-grafted membranes are more suitable for virus purification than the directly grafted ones."

Our news journalists obtained a quote from the research from the University Nova of Lisboa, "By reducing the number of grafted ligands to low (1.7 mu mol/cm(2)) or medium (2.4 mu mol/cm(2)) density, it is possible to increase the recovery of purified virus by 60% compared to a highly charged membrane (3.3 mu mol/cm(2)) that yielded a recovery rate lower than 30%. In the reported experiments, Sartobind() Q, chosen as benchmark comparison, provides a better compromise between high recovery and large dynamic binding capacity."

According to the news editors, the research concluded: "Overall, this work contributes to the understanding and development of new membrane adsorbers specifically designed for virus purification."

For more information on this research see: Impact of grafting on the design of new membrane adsorbers for adenovirus purification. Journal of Biotechnology, 2014;181():1-11. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Biotechnology - www.elsevier.com/wps/product/cws_home/505515)

The news correspondents report that additional information may be obtained from P. Nestola, Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept. of Quim, Requimte CQFB, P-2829516 Caparica, Portugal. Additional authors for this research include L. Villain, C. Peixoto, D.L. Martins, P.M. Alves, M.J.T. Carrondo and J.P.B. Mota (see also Adenovirus).

Keywords for this news article include: Europe, Viruses, Caparica, Portugal, Virology, Adenovirus

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Virus Weekly


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