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Investigators at Aarhus University Target Enzymes and Coenzymes

June 18, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news reporting out of Aarhus, Denmark, by NewsRx editors, research stated, "We report on the assembly of multi-layered polyelectrolyte thin films containing an immobilized enzyme to perform conversion of externally administered prodrugs and achieve delivery of the resulting therapeutics to adhering cells. Towards this goal, multi-layered coatings were assembled using poly(sodium styrene sulfonate) and poly(allylamine hydrochloride)."

Our news journalists obtained a quote from the research from Aarhus University, "Activity of the incorporated enzyme was quantified as a function of the assembly conditions, position of the enzyme within the multi-layered architecture, concentration of the enzyme in the adsorption solution, and concentration of the administered prodrug. Biocatalytic coatings exhibited sustained levels of enzymatic activity over at least one week of incubation in physiological buffers without signs of loss of activity of the enzyme. Developed enzyme-containing polymer films afforded zero-order release of the in situ synthesized cargo with kinetics of synthesis (nM per hour) covering at least 3 orders of magnitude. Internalization of the synthesized product by adhering cells was visualized using a fluorogenic enzyme substrate. Therapeutic utility of biocatalytic coatings was demonstrated using a myoblast cell line and a prodrug for the anti-proliferative agent, 5-fluorouridine."

According to the news editors, the research concluded: "Taken together, this work presents a novel approach to delivery of small molecule drugs using multi-layered polymer thin films with utility in surface-mediated drug delivery, assembly of therapeutic implantable devices, and tissue engineering."

For more information on this research see: Biocatalytic polymer thin films: optimization of the multilayered architecture towards in situ synthesis of anti-proliferative drugs. Nanoscale, 2014;6(8):4131-40. (Royal Society of Chemistry - www.rsc.org/; Nanoscale - pubs.rsc.org/en/journals/journalissues/nr)

Our news journalists report that additional information may be obtained by contacting S.0. Andreasen, Dept. of Chemistry, Aarhus University, Aarhus C 8000, Denmark. Additional authors for this research include B. Fejerskov and A.N Zelikin (see also Enzymes and Coenzymes).

Keywords for this news article include: Tissue Engineering, Biomedical Engineering, Biomedicine, Aarhus, Europe, Denmark, Bioengineering, Enzymes and Coenzymes.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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