By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Life Science Research. According to news reporting out of Porto, Portugal, by NewsRx editors, the research stated, "In this study, a microfluidic cross-slot device is used to examine the extensional flow response of diluted porcine synovial fluid (PSF) samples using flow-induced birefringence (FIB) measurements. The PSF sample is diluted to 10 x 20 x and 30 x its original mass in a phosphate-buffered saline and its FIB response measured as a function of the strain rate at the stagnation point of the cross-slots."
Our news journalists obtained a quote from the research from the University of Porto, "Equivalent experiments are also carried out using trypsin-treated PSF (t-PSF) in which the protein content is digested away using an enzyme. The results show that, at the synovial fluid concentrations tested, the protein content plays a negligible role in either the fluid's bulk shear or extensional flow behaviour. This helps support the validity of the analysis of synovial fluid HA content, either by microfluidic or by other techniques where the synovial fluid is first diluted, and suggests that the HA and protein content in synovial fluid must be higher than a certain minimum threshold concentration before HA-protein or protein-protein interactions become significant. However a systematic shift in the FIB response as the PSF and t-PSF samples are progressively diluted indicates that HA-HA interactions remain significant at the concentrations tested."
According to the news editors, the research concluded: "These interactions influence FIB-derived macromolecular parameters such as the relaxation time and the molecular weight distribution and therefore must be minimized for the best validity of this method as an analytical technique, in which non-interaction between molecules is assumed."
For more information on this research see: Synovial fluid response to extensional flow: effects of dilution and intermolecular interactions. Plos One, 2014;9(3):e92867. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news journalists report that additional information may be obtained by contacting S.J. Haward, Faculdade de Engenharia da Universidade do Porto, Departamento de Engenharia Quimica, Centro de Estudos de Fenomenos de Transporte, Porto, Portugal (see also Life Science Research).
Keywords for this news article include: Europe, Portugal, Life Science Research.
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