News Column

Findings from Shanghai Jiao-Tong University in the Area of Cancer Gene Therapy Reported

June 16, 2014

By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Current study results on Biotechnology have been published. According to news reporting out of Shanghai, People's Republic of China, by NewsRx editors, research stated, "Polyamidoamine dendrimer (PAMAM) is a new nanometer material, which can transfer the target genes to cells with high efficiency and lower toxicity. This study aims to evaluate antitumor effects of survivin antisense oligonucleotide (survivin-asODN) (carried by polyamidoamine dendrimer liposome) on hepatic cancer in nude mice."

Our news journalists obtained a quote from the research from Shanghai Jiao-Tong University, "Hepatic cancer model was established by injecting SMMC-7721 cells subcutaneously into flanks of nude mice. Polyamidoamine dendrimer and liposome were mixed with survivin-asODN, respectively. The shape and size of complex were observed by transmission electron microscope, and zeta potential was measured by an analytical tool. Encapsulation efficiency and DNA loading level were determined by an ultraviolet spectrophotometer in centrifuging method. Expression of survivin in transplant tumor was measured by Western blotting. No significant difference appeared for diameter and envelopment ratio between PAMAM liposome-survivin-asODN and PAMAM-survivin-asODN (P > 0.05). Both zeta potential and transfection efficiency in PAMAM liposome-survivin-asODN were higher than that in PAMAM-survivin-asODN complex (P < 0.05). Expression of survivin protein and weight of tumors in transplanted tumors in PAMAM liposome-survivin-asODN group was less than that in PAMAM-survivin-asODN group (P < 0.05). Cell apoptosis rate in PAMAM liposome-survivin-asODN group was higher than that of PAMAM-survivin-asODN group (P < 0.05)."

According to the news editors, the research concluded: "Polyamidoamine dendrimer liposome can deliver survivin-asODN into hepatic transplanted tumor cells effectively. Ployamidoamine dendrimer liposome-mediated survivin-asODN can inhibit hepatic cell proliferation by inducing apoptosis."

For more information on this research see: Polyamidoamine dendrimer liposome-mediated survivin antisense oligonucleotide inhibits hepatic cancer cell proliferation by inducing apoptosis. Tumor Biology, 2014;35(5):5013-5019. Tumor Biology can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer -; Tumor Biology -

Our news journalists report that additional information may be obtained by contacting S. Han, Shanghai Jiao Tong University, Res Inst Micro Nanometer Sci & Technol, Shanghai 200030, People's Republic of China. Additional authors for this research include Z. Cai, L. Peng, Z. Li, H.B. Zhou, X.Q. Li, S.Z. Fang, Z.H. Huang and D.X. Cui (see also Biotechnology).

Keywords for this news article include: Asia, Antisense Technology, Biotechnology, Shanghai, Genetics, Oncology, Apoptosis, Bioengineering, Cell Proliferation, Cancer Gene Therapy, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Cancer Gene Therapy Week

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