By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Proteins are discussed in a new report. According to news originating from Shanghai, People's Republic of China, by NewsRx correspondents, research stated, "Efficient enrichment of specific glycoproteins from complex biological samples is of great importance towards the discovery of disease biomarkers in biological systems. Recently, phenylboronic acid-based functional materials have been widely used for enrichment of glycoproteins."
Our news journalists obtained a quote from the research from Fudan University, "However, such enrichment was mainly carried out under alkaline conditions, which is different to the status of glycoproteins in neutral physiological conditions and may cause some unpredictable degradation. In this study, on-demand neutral enrichment of glycoproteins from crude biological samples is accomplished by utilizing the reversible interaction between the cis-diols of glycoproteins and benzoboroxole-functionalized magnetic composite microspheres (Fe3O4/PAA-AOPB). The Fe3O4/PAA-AOPB composite microspheres are deliberately designed and constructed with a high-magnetic-response magnetic supraparticle (MSP) core and a crosslinked poly(acrylic acid) (PAA) shell anchoring abundant benzoboroxole functional groups on the surface. These nanocomposites possessed many merits, such as large enrichment capacity (93.9 mg/g, protein/beads), low non-specific adsorption, quick enrichment process (10 min) and magnetic separation speed (20 s), and high recovery efficiency."
According to the news editors, the research concluded: "Furthermore, the as-prepared Fe3O4/PAA-AOPB microspheres display high selectivity to glycoproteins even in the E. coli lysate or fetal bovine serum, showing great potential in the identify of low-abundance glycoproteins as biomarkers in real complex biological systems for clinical diagnoses."
For more information on this research see: Benzoboroxole-Functionalized Magnetic Core/Shell Microspheres for Highly Specifi c Enrichment of Glycoproteins under Physiological Conditions. Small, 2014;10(7):1379-1386. Small can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell - www.wiley.com/; Small - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-6829)
The news correspondents report that additional information may be obtained from Y.T. Zhang, Fudan University, Adv Mat Lab, Shanghai 200433, People's Republic of China. Additional authors for this research include W.F. Ma, D. Li, M. Yu, J. Guo and C.C. Wang (see also Proteins).
Keywords for this news article include: Asia, Shanghai, Glycoproteins, Glycoconjugates, People's Republic of China
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