News Column

Findings from Cornell University College of Medicine in Virology Reported

June 19, 2014



By a News Reporter-Staff News Editor at Gene Therapy Weekly -- A new study on Life Science Research is now available. According to news originating from New York City, New York, by NewsRx correspondents, research stated, "Viral integrations are important in human biology, yet genome-wide integration profiles have not been determined for many viruses. Adeno-associated virus (AAV) infects most of the human population and is a prevalent gene therapy vector."

Our news journalists obtained a quote from the research from the Cornell University College of Medicine, "AAV integrates into the human genome with preference for a single locus, termed AAVS1. However, the genome-wide integration of AAV has not been defined, and the principles underlying this recombination remain unclear. Using a novel high-throughput approach, integrant capture sequencing, nearly 12 million AAV junctions were recovered from a human cell line, providing five orders of magnitude more data than were previously available. Forty-five percent of integrations occurred near AAVS1, and several thousand novel integration hotspots were identified computationally. Most of these occurred in genes, with dozens of hotspots targeting known oncogenes. Viral replication protein binding sites (RBS) and transcriptional activity were major factors favoring integration. In a first for eukaryotic viruses, the data reveal a unique asymmetric integration profile with distinctive directional orientation of viral genomes."

According to the news editors, the research concluded: "These studies provide a new understanding of AAV integration biology through the use of unbiased high-throughput data acquisition and bioinformatics."

For more information on this research see: High-throughput sequencing reveals principles of adeno-associated virus serotype 2 integration. The Journal of Virology, 2013;87(15):8559-68 (see also Life Science Research).

The news correspondents report that additional information may be obtained from T. Janovitz, Tri-Institutional MD-PhD Program, Weill Medical College of Cornell University, New York, New York, United States. Additional authors for this research include I.A. Klein, T. Oliveira, P. Mukherjee, M.C. Nussenzweig, M. Sadelain and E. Falck-Pedersen.

Keywords for this news article include: Biotechnology, Viral, Viruses, Virology, Gene Therapy, New York City, United States, Bioengineering, Life Science Research, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Gene Therapy Weekly


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