News Column

University of Saarland Details Findings in Nanoparticles

May 16, 2014

By a News Reporter-Staff News Editor at Science Letter -- Current study results on Nanoparticles have been published. According to news reporting originating from Saarbrucken, Germany, by NewsRx correspondents, research stated, "Fluorescently labeled nanoparticles are widely used to investigate nanoparticle cell interactions by fluorescence microscopy. Owing to limited lateral and axial resolution, nanostructures (

Our news editors obtained a quote from the research from the University of Saarland, "Especially after uptake into cells, a common fate of the fluorescence label and the particle core cannot be taken for granted. In this study, a correlative approach is presented to image fluorescently labeled gold nanoparticles inside whole cells by correlative light and electron microscopy (CLEM). This approach allows for detection of the fluorescently labeled particle shell as well as for the gold core in one sample. In this setup, A549 cells are exposed to 8 nm Atto 647N-labeled gold nanoparticles (3.3 x 10(9) particles mL(-1), 0.02 mu g Au mL(-1)) for 5 h and are subsequently imaged by confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). Eight fluorescence signals located at different intracellular positions are further analyzed by TEM. Five of the eight fluorescence spots are correlated with isolated or agglomerated gold nanoparticles."

According to the news editors, the research concluded: "Three fluorescence signals could not be related to the presence of gold, indicating a loss of the particle shell."

For more information on this research see: A Correlative Analysis of Gold Nanoparticles Internalized by A549 Cells. Particle & Particle Systems Characterization, 2014;31(4):439-448. Particle & Particle Systems Characterization can be contacted at: Wiley-V C H Verlag Gmbh, Boschstrasse 12, D-69469 Weinheim, Germany. (Wiley-Blackwell -; Particle & Particle Systems Characterization -

The news editors report that additional information may be obtained by contacting K. Bose, University of Saarland, D-66123 Saarbrucken, Germany. Additional authors for this research include M. Koch, C. Cavelius, A.K. Kiemer and A. Kraegeloh (see also Nanoparticles).

Keywords for this news article include: Europe, Germany, Saarbrucken, Nanotechnology, Emerging Technologies

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

For more stories covering the world of technology, please see HispanicBusiness' Tech Channel

Source: Science Letter

Story Tools Facebook Linkedin Twitter RSS Feed Email Alerts & Newsletters