News Column

New Findings from Stanford University in DNA Research Provides New Insights

May 16, 2014

By a News Reporter-Staff News Editor at Science Letter -- Research findings on DNA Research are discussed in a new report. According to news reporting out of Stanford, California, by NewsRx editors, research stated, "Single-molecule measurements of DNA twist and extension have been used to reveal physical properties of the double helix and to characterize structural dynamics and mechanochemistry in nucleoprotein complexes. However, the spatiotemporal resolution of twist measurements has been limited by the use of angular probes with high rotational drag, which prevents detection of short-lived intermediates or small angular steps."

Our news journalists obtained a quote from the research from Stanford University, "We introduce gold rotor bead tracking (AuRBT), which yields >100x improvement in time resolution over previous techniques. AuRBT employs gold nanoparticles as bright low-drag rotational and extensional probes, which are monitored by instrumentation that combines magnetic tweezers with objective-side evanescent darkfield microscopy. Our analysis of high-speed structural dynamics of DNA gyrase using AuRBT revealed an unanticipated transient intermediate."

According to the news editors, the research concluded: "AuRBT also enables direct measurements of DNA torque with >50x shorter integration times than previous techniques; we demonstrated high-resolution torque spectroscopy by mapping the conformational landscape of a Z-forming DNA sequence."

For more information on this research see: Gold rotor bead tracking for high-speed measurements of DNA twist, torque and extension. Nature Methods, 2014;11(4):456-462,148-149. Nature Methods can be contacted at: Nature Publishing Group, Macmillan Building, 4 Crinan St, London N1 9XW, England. (Nature Publishing Group -; Nature Methods -

Our news journalists report that additional information may be obtained by contacting P. Lebel, Stanford University, Medical Center, Dept. of Biol Struct, Stanford, CA 94305, United States. Additional authors for this research include A. Basu, F.C. Oberstrass, E.M. Tretter and Z. Bryant (see also DNA Research).

Keywords for this news article include: Stanford, California, DNA Research, United States, North and Central America

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Source: Science Letter

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