News Column

Studies from University of Illinois Yield New Data on Nanoparticles

May 14, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Nanoparticles have been presented. According to news reporting originating from Urbana, Illinois, by NewsRx correspondents, research stated, "Iron deficiency anemia afflicts 1 in 3 individuals, mostly women and children worldwide. A novel application using iron-oxide nanoparticles (IONPs) and a photonic crystal (PC) optical biosensor as an immunodiagnostic platform for detection of serum ferritin, a biomarker for iron deficiency, is presented."

Our news editors obtained a quote from the research from the University of Illinois, "Human liver ferritin (450 kDa), clinical serum controls, and three commercially available ferritin ELISA tests were used to evaluate the PC biosensor assay in terms of inter- and intra-assay variability, spike-recovery (%), limit of detection CLOD), and matrix effects on binding. For the PC biosensor, signal response from label-free, sandwich with secondary antibody (pAb), and pAb functionalized with iron-oxide nanoparticles (FpAb) assays were detected using the Biomolecular Interaction Detection (BIND) system. Bland-Altman analysis was used to evaluate agreement between expected values for ferritin in control sera and each of the detection platforms. Inter- and intra-assay variability of the PC biosensor were both < 10%. Percent mean recovery ( +/- %RSD) of ferritin from two control sera samples were 94.3% (13.1%) and 96.9% (7.6%). Use of FpAb in PC biosensor resulted in two orders of magnitude increase in sensitivity compared to label-free assay; capable of measuring serum ferritin as low as 26 ng/mL. In comparison to ELISA tests, the PC biosensor assay had the lowest bias ( -1.26; 95% Cl [-3.0-5.51) and narrower limit of agreement (-11.6-9.1 ng/mL) when determining ferritin concentrations from control sera."

According to the news editors, the research concluded: "These proof-of-concept studies support the use of IONPs to enhance detection sensitivity of PC biosensors for determination of biomarkers of nutritional status."

For more information on this research see: A photonic crystal biosensor assay for ferritin utilizing iron-oxide nanoparticles. Biosensors & Bioelectronics, 2014;56():320-327. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)

The news editors report that additional information may be obtained by contacting R.D. Peterson, University of Illinois, Div Nutr Sci, Urbana, IL 61801, United States. Additional authors for this research include B.T. Cunningham and J.E. Andrade (see also Nanoparticles).

Keywords for this news article include: Urbana, Illinois, Biosensing, United States, Bioengineering, Bionanotechnology, Nanobiotechnology, Emerging Technologies, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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