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Studies from Tsinghua University Have Provided New Data on Blood Coagulation Factors

May 14, 2014

By a News Reporter-Staff News Editor at Biotech Week -- A new study on Hematology is now available. According to news reporting originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "In this work, a convenient and high-throughput colorimetric assay was developed on an aptamer-modified microchip for ultrasensitive detection of thrombin using rolling circle amplification and G-quadruplex DNAzyme. This system consisted of an aptamer-modified microchip and a secondary aptamer."

Our news editors obtained a quote from the research from Tsinghua University, "The secondary aptamer contained a thrombin aptamer and a primer with a G-quadruplex circular template. RCA technology was used to improve the sensitivity by producing the multiple G-quadruplex units. To generate colorimetric signal, G-quadruplex DNAzyme was used to catalyze the H2O2-mediated oxidation of 2,2'-azinobis (3-ethylbenzothiozoline)-6-sulfonic acid. At the optimal conditions, the linear range for thrombin was 0.100-50.000 pg/mL, and the limit of detection was down to 0.083 pg/mL."

According to the news editors, the research concluded: "Moreover, the developed method was successfully applied to detect thrombin from human plasma and serum, indicating that this approach has great potential in clinical diagnosis and medical investigation."

For more information on this research see: A portable microchip for ultrasensitive and high-throughput assay of thrombin by rolling circle amplification and hemin/G-quadruplex system. Biosensors & Bioelectronics, 2014;56():71-76. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier -; Biosensors & Bioelectronics -

The news editors report that additional information may be obtained by contacting X.X. Lin, Tsinghua Univ, Beijing Key Lab Microanalyt Methods & Instrumenta, Dept. of Chem, Beijing 100084, People's Republic of China. Additional authors for this research include Q.S. Chen, W. Liu, H.F. Li and J.M. Lin (see also Hematology).

Keywords for this news article include: Asia, Heme, Drugs, Hemin, Beijing, Therapy, Thrombin, Hematology, Porphyrins, Biological Factors, Peptide Hydrolases, Enzymes and Coenzymes, Serine Endopeptidases, Blood Coagulation Factors, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Biotech Week

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