By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Biomedicine and Biomedical Engineering are discussed in a new report. According to news reporting from Jerusalem, Israel, by NewsRx journalists, research stated, "Many cell lines, despite the fact that they are easy to culture, tend to lose some of their in vivo characteristics in vitro, we therefore decided to investigate whether culturing HK-2 cells on kidney derived micro-scaffolds (KMS) could improve proximal tubule functionality to these cells. Kidney derived micro-scaffolds (KMS) have been prepared that, due to the fact that they are only 300 m in depth, allow for transfer of gases and nutrients via diffusion whilst maintaining the kidney's intricate microstructure."
The news correspondents obtained a quote from the research from Hebrew University, "Culturing HK-2 on KMS shows significant increase in expression of AQP-1, ATP1B1, SLC23A1 and SLC5A2 after 1, 2 and 3 weeks compared with HK-2 grown under standard tissue culture conditions. Additionally, very high levels of expression of CCL-2 (15-30 fold increase) and LRP-2 (25-200 fold increase) were observed when the HK-2 were grown on KMS compared with HK-2 grown under standard tissue culture conditions. Furthermore, HK-2 cells grown under standard conditions released higher levels of Il-6 and Il-8 compared with primary tubule cells (Asterand AS-9-2) and secreted no MCP-1 or RANTES as opposed to primary cells that released MCP-1 and RANTES following stimulation. However, HK-2 grown on KMS showed both a marked decrease in Il-6/Il-8 secretion in line with the primary cells and secreted MCP-1 as well."
According to the news reporters, the research concluded: "These results show that the micro-environment of the KMS assists in restoring in vivo like properties to the HK-2 cells."
For more information on this research see: Kidney derived micro-scaffolds enable HK-2 cells to develop more in-vivo like properties. Experimental Cell Research, 2014;322(1):71-80. (Elsevier - www.elsevier.com; Experimental Cell Research - www.elsevier.com/wps/product/cws_home/622826)
Our news journalists report that additional information may be obtained by contacting G. Finesilver, Dept. of Cellular & Developmental Biology, Hebrew University, Jerusalem, Israel. Additional authors for this research include J. Bailly, M. Kahana and E. Mitrani (see also Biomedicine and Biomedical Engineering).
Keywords for this news article include: Asia, Tissue Engineering, Biomedicine and Biomedical Engineering, Israel, Jerusalem, Bioengineering.
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