News Column

Reports Outline Immunoglobulins Study Results from Central South University

May 13, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Immunology. According to news reporting originating in Changsha, People's Republic of China, by NewsRx journalists, research stated, "Ferrocene (Fc) functionalized self-assembled peptide nanowire (Fc-PNW) was synthesized, functionalized and used as label for the fabrication of electrochemical immunosensors to detect substance P (SP). To prepare the label, gold nanoparticles (AuNPs) were immobilized onto the Fc-PNW for the following adsorption of horseradish peroxidase (HRP) and secondary anti-SP antibody (Ab(2), Fc-PNW-HRP-Ab(2))."

The news reporters obtained a quote from the research from Central South University, "The role of Fc-PNW in the electrochemical label was not only as supporting matrix for HRP and Ab(2), but also as the mediator for HRP to enhance the sensitivity of HRP toward H2O2 detection. The immunosensor was fabricated based on the traditional sandwich protocol with primary anti-SP antibody (Ab(1)) immobilized onto graphene surface. The catalytic current of HRP toward H2O2 in the presence of mediator Fc was used as signal of the immunosensor. Different parameters, such as the weight ratio of HRP to Ab(2) on the Fc-PNW and the antibody-antigen incubation time were optimized to improve the performance of the immunosensor."

According to the news reporters, the research concluded: "The resulting immunosensor has high sensitivity, wide linear range (0.01-50 ng/mL) and low detection limit (5 pg/mL)."

For more information on this research see: Enzyme modified peptide nanowire as label for the fabrication of electrochemical immunosensor. Sensors and Actuators B-Chemical, 2014;196():189-193. Sensors and Actuators B-Chemical can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also Immunology).

Our news correspondents report that additional information may be obtained by contacting J.L. Zhang, Central South University, Coll Chem & Chem Engn, Changsha 410008, Hunan, People's Republic of China. Additional authors for this research include X. Chen and M.H. Yang.

Keywords for this news article include: Asia, Antibodies, Changsha, Peptides, Chemistry, Immunology, Proteomics, Blood Proteins, Electrochemical, Immunoglobulins, People's Republic of China

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Source: Life Science Weekly

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