News Column

Recent Findings from Aaron Diamond AIDS Research Center Advance Knowledge in Gene Therapy

May 15, 2014



By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Data detailed on Biotechnology have been presented. According to news originating from New York City, New York, by NewsRx correspondents, research stated, "In the present study, a novel adeno-associated virus (AAV) vector-mediated gene delivery approach was taken to improve the reconstitution of functional CD8(+) T cells in humanized mice, thereby mimicking the human immune system (HIS). Human genes encoding HLA-A2 and selected human cytokines (A2/hucytokines) were introduced to an immune-deficient mouse model [NOD/SCID/IL2r?(null) (NSG) mice] using AAV serotype 9 (AAV9) vectors, followed by transplantation of human hematopoietic stem cells."

Our news journalists obtained a quote from the research from Aaron Diamond AIDS Research Center, "NSG mice transduced with AAV9 encoding A2/hucytokines resulted in higher levels of reconstitution of human CD45(+) cells compared to NSG mice transduced with AAV9 encoding HLA-A2 alone or HLA-A2-transgenic NSG mice. Furthermore, this group of HIS mice also mounted the highest level of antigen-specific A2-restricted human CD8(+) T-cell response upon vaccination with recombinant adenoviruses expressing human malaria and HIV antigens. Finally, the human CD8(+) T-cell response induced in human malaria vaccine-immunized HIS mice was shown to be functional by displaying cytotoxic activity against hepatocytes that express the human malaria antigen in the context of A2 molecules. Taken together, our data show that AAV vector-mediated gene delivery is a simple and efficient method to transfer multiple human genes to immune-deficient mice, thus facilitating successful reconstitution of HIS in mice."

According to the news editors, the research concluded: "The HIS mice generated in this study should ultimately allow us to swiftly evaluate the T-cell immunogenicity of various human vaccine candidates in a pre-clinical setting."

For more information on this research see: An AAV vector-mediated gene delivery approach facilitates reconstitution of functional human CD8+ T cells in mice. Plos One, 2014;9(2):e88205. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)

The news correspondents report that additional information may be obtained from J. Huang, HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New York, New York, United States. Additional authors for this research include X. Li, J.G. Coelho-dos-Reis, J.M. Wilson and M. Tsuji (see also Biotechnology).

Keywords for this news article include: Biotechnology, Genetics, Immunology, CD Antigens, CD8 Antigens, Gene Therapy, New York City, United States, Bioengineering, Differentiation, Malaria Vaccines, Biological Factors, Protozoan Vaccines, Biological Products, Protozoan Infections, T Lymphocyte Antigens, North and Central America.

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Source: Gene Therapy Weekly