By a News Reporter-Staff News Editor at Biotech Week -- A new study on Quantum Dots is now available. According to news reporting out of Chongqing, People's Republic of China, by NewsRx editors, research stated, "Mycotoxins are fatal threats in food safety due to their strong carcinogenesis and toxicity, thus requiring highly sensitive detections. Herein, different-sized quantum dots (QDs) were used to construct a Forster resonance energy transfer (FRET) based immunosensor for sensitive detection of aflatoxin B-1 (AFB(1)) in rice grains."
Our news journalists obtained a quote from the research from Southwest University, "To avoid irregular aggregation between two kinds of QDs, monovalent monoclonal antibody (mAb)-labeled red QDs (similar to 0.84 anti-AFB(1) mAbs per QD) and multivalent hapten-labeled green QDs (similar to 6.8 AFB(1) per QD) were designed as acceptor and donor, respectively. The anti-AFB(1) mAbs and AFB(1) interactions promoted one or more acceptors bound with a multivalent AFB(1)-labeled donor, resulting in energy transfer from the green QDs to the red QDs. Various parameters that influence the immunoassay including reactant ratio of donor to acceptor, buffer pH value, buffer ionic strength and immunoreaction time were systematically investigated and optimized. With optimal conditions, the obtained energy transfer efficiency is proportional to the logarithm of AFB(1) concentration in a range over 0.19-16 pM (0.06-5 ng/mL), while offering a limit of detection of 0.13 pM (0.04 ng/mL) in rice extracts. The recovery rates of the intra-assay for spiked samples at AFB(1) concentrations of 0.1, 1.0, and 5.0 ng/mL were 83.27% +/- 3.27%, 97.36% +/- 4.55% and 83.04% +/- 4.94%, respectively, and those for the inter-assay were 81.28% +/- 6.11%, 95.97% +/- 7.07%, and 82.78% +/- 5.99%, respectively."
According to the news editors, the research concluded: "Statistical analysis using t-test had no significant difference between the proposed FRET-based immunoassay and the commercial enzyme-linked immunosorbent assay kit."
For more information on this research see: A homogeneous immunosensor for AFB(1) detection based on FRET between different-sized quantum dots. Biosensors & Bioelectronics, 2014;56():144-150. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news journalists report that additional information may be obtained by contacting W. Xu, Southwest University, ICEAM, Chongqing 400715, People's Republic of China. Additional authors for this research include Y.H. Xiong, W.H. Lai, Y. Xu, C.M. Li and M.Y. Xie (see also Quantum Dots).
Keywords for this news article include: Asia, Chongqing, Quantum Dots, Nanotechnology, Quantum Physics, Emerging Technologies, People's Republic of China
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