News Column

Findings from University of Toronto in Immunoglobulins Reported

May 14, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Immunology have been published. According to news reporting out of Toronto, Canada, by NewsRx editors, research stated, "In order to comprehensively manipulate the human proteome we require a vast repertoire of pharmacological reagents. To address these needs we have developed repertoires of synthetic antibodies by phage display, where diversified oligonucleotides are used to modify the complementarity-determining regions (CDRs) of a human antigen-binding fragment (Fab) scaffold."

Our news journalists obtained a quote from the research from the University of Toronto, "As diversity is produced outside the confines of the mammalian immune system, synthetic antibody libraries allow us to bypass several limitations of hybridoma technology while improving the experimental parameters under which pharmacological reagents are produced. Here we describe the methodologies used to produce synthetic antibody libraries from a single human framework with diversity restricted to four CDRs. These synthetic repertoires can be extremely functional as they produce highly selective, high affinity Fabs to the majority of soluble human antigens. Finally we describe selection methodologies that allow us to overcome immuno-dominance in our selections to target a variety of epitopes per antigen."

According to the news editors, the research concluded: "Together these methodologies allow us to produce human monoclonal antibodies to manipulate the human proteome."

For more information on this research see: Recombinant genetic libraries and human monoclonal antibodies. Methods In Molecular Biology, 2014;1060():149-70 (see also Immunology).

Our news journalists report that additional information may be obtained by contacting J.J. Adams, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada. Additional authors for this research include B. Nelson and S.S Sidhu.

Keywords for this news article include: Canada, Toronto, Ontario, Genetics, Immunology, Blood Proteins, Immunoglobulins, Serum Globulins, Monoclonal Antibodies, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Biotech Week

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