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Data from University of Navarra Advance Knowledge in Stem Cells

May 14, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Stem Cell Research. According to news originating from Pamplona, Spain, by NewsRx correspondents, research stated, "Due to the attractive properties of poly(L-lactic acid) (PLLA) for tissue engineering, the aim was to determine the growth and differentiation capacity of mesenchymal stromal cells (MSCs) in PLLA scaffolds and their potential use in the treatment of cartilage diseases. MSCs were cultured in PLLA films and thin porous membranes to study adherence and proliferation."

Our news journalists obtained a quote from the research from the University of Navarra, "Permeability and porosity were determined for the different scaffolds employed. The optimal conditions for cell seeding were first determined, as well as cell density and distribution inside the PLLA. Scaffolds were then maintained in expansion or chondrogenic differentiation media for 21 days. Apoptosis, proliferation and chondrogenic differentiation was assessed after 21 days in culture by immunohistochemistry. Mechanical characteristics of scaffolds were determined before and after cell seeding. MSCs uniformly adhered to PLLA films as well as to porous membranes. Proliferation was detected only in monolayers of pure PLLA, but was no longer detected after 10 days. Mechanical characterization of PLLA scaffolds showed differences in the apparent compression elastic modulus for the two sizes used. After determining high efficiencies of seeding, the production of extracellular matrix (ECM) was determined and contained aggrecan and collagens type I and X. ECM produced by the cells induced a twofold increase in the apparent elastic modulus of the composite. Biocompatible PLLA scaffolds have been developed that can be efficiently loaded with MSCs. The scaffold supports chondrogenic differentiation and ECM deposition that improves the mechanics of the scaffold."

According to the news editors, the research concluded: "Although this improvement does not met the expectations of a hyaline-like cartilage ECM, in part due to the lack of a mechanical stimulation, their potential use in the treatment of cartilage pathologies encourages to improve the mechanical component."

For more information on this research see: Culture of human bone marrow-derived mesenchymal stem cells on of poly(L-lactic acid) scaffolds: potential application for the tissue engineering of cartilage. Knee Surgery, Sports Traumatology, Arthroscopy, 2013;21(8):1737-50. Knee Surgery, Sports Traumatology, Arthroscopy can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer -; Knee Surgery, Sports Traumatology, Arthroscopy -

The news correspondents report that additional information may be obtained from I. Izal, Laboratory for Orthopaedic Research, University of Navarra, Irunlarrea, 1, 31080, Pamplona, Spain. Additional authors for this research include P. Aranda, P. Sanz-Ramos, P. Ripalda, G. Mora, F. Granero-Molto, H. Deplaine, J.L. Gomez-Ribelles, G.G. Ferrer, V. Acosta, I. Ochoa, J.M. Garcia-Aznar, E.J. Andreu, M. Monleon-Pradas, M. Doblare and F. Prosper (see also Stem Cell Research).

The publisher's contact information for the journal Knee Surgery, Sports Traumatology, Arthroscopy is: Springer, 233 Spring Street, New York, NY 10013, USA.

Keywords for this news article include: Tissue Engineering, Biomedical Engineering, Biomedicine, Spain, Europe, Pamplona, Bone Marrow, Bone Research, Immune System, Bioengineering, Stem Cell Research, Mesenchymal Stem Cells.

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Source: Biotech Week

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