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Recent Findings from H.Y. Jiang and Co-Authors Provide New Insights into Chemiluminescence

June 6, 2014



By a News Reporter-Staff News Editor at Science Letter -- Current study results on Chemiluminescence have been published. According to news originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "A chemiluminescent enzyme immunoassay (CLEIA) was compared to an ultraperformance liquid chromatography tandem mass spectroscopy (UPLC-MS/MS) procedure for the analysis of zeranol and its metabolites in bovine tissue samples. Apparent recoveries from fortified samples by both methods were comparable at 0.5-4.0 mu g/kg and a significant correlation was obtained."

Our news journalists obtained a quote from the research, "For CLEIA analysis, hapten mimicking the analyte was first synthesized and conjugated with the carrier protein bovine serum albumin as the immunogen to produce monoclonal antibody. The obtained antibody showed extensive cross-reactivity toward zeranol metabolites (zearalanone). The limit of detection of CLEIA and UPLC-MS/MS was 0.05 mu g/kg and 0.5 mu g/kg, respectively. Recoveries of both methods for fortified samples were higher than 75.0% with the coefficient of variation less than 15%."

According to the news editors, the research concluded: "These results indicated that the combination of screening with CLEIA and confirmation with UPLC-MS/MS for zeranol and its metabolites would be a reliable method for a large number of bovine samples."

For more information on this research see: Determination of zeranol and its metabolites in bovine muscle and liver by a chemiluminescence enzyme immunoassay: compared to an ultraperformance liquid chromatography tandem mass spectroscopy method. Luminescence, 2014;29(4):393-400. Luminescence can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Luminescence - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1522-7243)

The news correspondents report that additional information may be obtained from H.Y. Jiang, Beijing WDWK Biotech Co Ltd, Beijing 100085, People's Republic of China. Additional authors for this research include W.J. Wang, J.H. Zhu, X.Q. Tao, J.C. Li, X. Xia, K. Wen, F. Xu, Z.P. Wang, M. Chen, X.M. Li, X.P. Wu, S.E. Wang and S.Y. Ding (see also Chemiluminescence).

Keywords for this news article include: Asia, Antibodies, Beijing, Immunology, Blood Proteins, Immunoglobulins, Chemiluminescence, Enzymes and Coenzymes, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Science Letter


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