News Column

Recent Findings from Center for Nanotechnology Advance Knowledge in Cellular Structures

June 6, 2014

By a News Reporter-Staff News Editor at Science Letter -- Fresh data on Cellular Structures are presented in a new report. According to news originating from Pisa, Italy, by NewsRx correspondents, research stated, "Spatial distribution and dynamics of plasma-membrane proteins are thought to be modulated by lipid composition and by the underlying cytoskeleton, which forms transient barriers to diffusion. So far this idea was probed by single-particle tracking of membrane components in which gold particles or antibodies were used to individually monitor the molecules of interest."

Our news journalists obtained a quote from the research from Center for Nanotechnology, "Unfortunately, the relatively large particles needed for single-particle tracking can in principle alter the very dynamics under study. Here, we use a method that makes it possible to investigate plasma-membrane proteins by means of small molecular labels, specifically single GFP constructs. First, fast imaging of the region of interest on the membrane is performed. For each time delay in the resulting stack of images the average spatial correlation function is calculated. We show that by fitting the series of correlation functions, the actual protein 'diffusion law' can be obtained directly from imaging, in the form of a mean-square displacement vs. time-delay plot, with no need for interpretative models. This approach is tested with several simulated 2D diffusion conditions and in live Chinese hamster ovary cells with a GFP-tagged transmembrane transferrin receptor, a well-known benchmark of membrane-skeleton-dependent transiently confined diffusion. This approach does not require extraction of the individual trajectories and can be used also with dim and dense molecules."

According to the news editors, the research concluded: "We argue that it represents a powerful tool for the determination of kinetic and thermodynamic parameters over very wide spatial and temporal scales."

For more information on this research see: Fast spatiotemporal correlation spectroscopy to determine protein lateral diffusion laws in live cell membranes. Proceedings of the National Academy of Sciences of the United States of America, 2013;110(30):12307-12. (National Academy of Sciences -; Proceedings of the National Academy of Sciences of the United States of America -

The news correspondents report that additional information may be obtained from C. Di Rienzo, Center for Nanotechnology Innovation at National Enterprise for nanoScience and nanoTechnology, Istituto Italiano di Tecnologia, 56127 Pisa, Italy. Additional authors for this research include E. Gratton, F. Beltram and F. Cardarelli (see also Cellular Structures).

Keywords for this news article include: Pisa, Italy, Europe, Cell Membrane, Cellular Structures.

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Source: Science Letter

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