News Column

Findings in Materials Science Reported from Chonbuk National University School of Medicine

June 3, 2014



By a News Reporter-Staff News Editor at Journal of Technology -- A new study on Materials Science is now available. According to news reporting originating from Jeonju, South Korea, by VerticalNews correspondents, research stated, "Modified chitosan nanoparticles were conveniently obtained by a one-step aerosol method, and their potential for gene transfection was investigated. Droplets containing modified chitosans were formed by collison atomization, dried to form solid particles, and collected and studied for potential use as nanocarriers."

Our news editors obtained a quote from the research from the Chonbuk National University School of Medicine, "Modified chitosans consisted of a chitosan backbone and an additional component [covalently attached cholesterol; or blends with poly(L-lysine) (PLL), polyethyleneimine (PEI), or poly(ethylene glycol) (PEG)]. Agarose gel retardation assays confirmed that modified chitosans could associate with plasmid DNA. Even though the average cell viability of cholesterol-chitosan (Ch-Cs) showed a slightly higher cytotoxicity (similar to 90% viability) than that for unmodified chitosan (Cs, similar to 95%), transfection (>7.5 x 10(5) in relative light units (RLU) mg(-1)) was more effective than it was for Cs (similar to 7.6 x 10(4) RLU mg(-1)). The blending of PEI with Cs (i.e., a Cs/PEI) to produce transfection complexes enhanced the transfection efficiency (similar to 1.3 x 10(6) RLU mg(-1)) more than did the addition of PLL (i.e., a Cs/PLL, similar to 9.3 x 10(5) RLU mg(-1)); however, it also resulted in higher cytotoxicity (similar to 86% viability for Cs/PEI vs similar to 94% for Cs/PLL). The average cell viability (similar to 92%) and transfection efficiency (similar to 1.9 x 10(6) RLU mg(-1)) were complemented further by addition of PEG in Cs/PEI complexes (i.e., a Cs/PEI-PEG)."

According to the news editors, the research concluded: "This work concludes that gene transfection of Cs can be significantly enhanced by adding cationic polymers during aerosol fabrication without wet chemical modification processes of Cs."

For more information on this research see: Aerosol-Based Fabrication of Modified Chitosans and Their Application for Gene Transfection. ACS Applied Materials & Interfaces, 2014;6(7):4597-4602. ACS Applied Materials & Interfaces can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Applied Materials & Interfaces - www.pubs.acs.org/journal/aamick)

The news editors report that additional information may be obtained by contacting J.H. Byeon, Chonbuk Natl Univ Med Sch & Hosp, Biomed Res Inst, Mol Imaging & Therapeut Med Res Center, Dept. of Nucl Med, Jeonju 561712, South Korea. Additional authors for this research include H.K. Kim, D.H. Thompson and J.T. Roberts.

Keywords for this news article include: Asia, Jeonju, South Korea, Materials Science

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Journal of Technology


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