News Column

University of Copenhagen Details Findings in Enzymes and Coenzymes

June 3, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news reporting originating from Copenhagen, Denmark, by NewsRx correspondents, research stated, "Employing viruses as nanoscopic lipid-enveloped test tubes allows the miniaturization of protein-protein interaction (PPI) assays while preserving the physiological environment necessary for particular biological processes. Applied to the study of the human immunodeficiency virus type 1 (HIV-1), viral biology and pathology can also be investigated in novel ways, both in vitro as well as in infected cells."

Our news editors obtained a quote from the research from the University of Copenhagen, "In this work we report on an experimental strategy that makes use of engineered HIV-1 viral particles, to allow for probing PPIs of the HIV-1 integrase (IN) inside viruses with single-molecule Forster resonance energy transfer (FRET) using fluorescent proteins (FP). We show that infectious fluorescently labeled viruses can be obtained and that the quantity of labels can be accurately measured and controlled inside individual viral particles. We demonstrate, with proper control experiments, the formation of IN oligomers in single viral particles and inside viral complexes in infected cells. Finally, we show a clear effect on IN oligomerization of small molecule inhibitors of interactions of IN with its natural human cofactor LEDGF/p75, corroborating that IN oligomer enhancing drugs are active already at the level of the virus and strongly suggesting the presence of a dynamic, enhanceable equilibrium between the IN dimer and tetramer in viral particles."

According to the news editors, the research concluded: "Although applied to the HIV-1 IN enzyme, our methodology for utilizing HIV virions as nanoscopic test tubes for probing PPIs is generic, i.e., other PPIs targeted into the HIV-1, or PPIs targeted into other viruses, can potentially be studied with a similar strategy."

For more information on this research see: HIV Virions as Nanoscopic Test Tubes for Probing Oligomerization of the Integrase Enzyme. ACS Nano, 2014;8(4):3531-3545. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society -; ACS Nano -

The news editors report that additional information may be obtained by contacting D. Borrenberghs, University of Copenhagen, Dept. of Chem, Nanosci Center, DK-2100 Copenhagen, Denmark. Additional authors for this research include W. Thys, S. Rocha, J. Demeulemeester, C. Weydert, P. Dedecker, J. Hofkens, Z. Debyser and J. Hendrix (see also Enzymes and Coenzymes).

Keywords for this news article include: Viral, Europe, Denmark, Viruses, Virology, Copenhagen, Enzymes and Coenzymes

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Source: Life Science Weekly

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