By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Inflammation. According to news reporting originating from Gangwon Do, South Korea, by NewsRx correspondents, research stated, "Paraoxonase 1 (PON1) is an antioxidant enzyme which plays a central role in various diseases. However, the mechanism and function of PON1 protein in inflammation are poorly understood."
Our news editors obtained a quote from the research from Hallym University, "Since PON1 protein alone cannot be delivered into cells, we generated a cell permeable PEP-1-PON1 protein using protein transduction domains, and examined whether it can protect against cell death in lipopolysaccharide (LPS) or hydrogen peroxide (H2O2)-treated Raw 264.7 cells as well as mice with 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced skin inflammation. We demonstrated that PEP-1-PON1 protein transduced into Raw 264.7 cells and markedly protected against LPS or H2O2-induced cell death by inhibiting cellular reactive oxygen species (ROS) levels, the inflammatory mediator's expression, activation of mitogen-activated protein kinases (MAPKs) and cellular apoptosis. Furthermore, topically applied PEP-1-PON1 protein ameliorates TPA-treated mice skin inflammation via a reduction of inflammatory response. Our results indicate that PEP-1-PON1 protein plays a key role in inflammation and oxidative stress in vitro and in vivo."
According to the news editors, the research concluded: "Therefore, we suggest that PEP-1-PON1 protein may provide a potential protein therapy against oxidative stress and inflammation."
For more information on this research see: PEP-1-PON1 protein regulates inflammatory response in raw 264.7 macrophages and ameliorates inflammation in a TPA-induced animal model. Plos One, 2014;9(1):e86034. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
The news editors report that additional information may be obtained by contacting M.J. Kim, Dept. of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon, Gangwondo, South Korea. Additional authors for this research include H.J. Jeong, D.W. Kim, E.J. Sohn, H.S. Jo, D.S. Kim, H.A. Kim, E.Y. Park, J.H. Park, O. Son, K.H. Han, J. Park, W.S. Eum and S.Y Choi (see also Inflammation).
Keywords for this news article include: Asia, Gangwon Do, Immunology, South Korea, Macrophages, Inflammation, Myeloid Cells, Connective Tissue Cells, Mononuclear Phagocyte System.
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