By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Proteins. According to news reporting out of St. Louis, Missouri, by NewsRx editors, research stated, "Electrochemical enzyme-linked lectinsorbent assays (ELLA) were developed using nanoporous gold (NPG) as a solid support for protein immobilization and as an electrode for the electrochemical determination of the product of the reaction between alkaline phosphatase (ALP) and p-aminophenyl phosphate (p-APP), which is p-aminophenol (p-AP). Glycoproteins or concanavalin A (Con A) and ALP conjugates were covalently immobilized onto lipoic acid self-assembled monolayers on NPG."
Our news journalists obtained a quote from the research from the University of Missouri, "The binding of Con A-ALP (or soybean agglutinin-ALP) conjugate to glycoproteins covalently immobilized on NPG and subsequent incubation with p-APP substrate was found to result in square-wave voltammograms whose peak difference current varied with the identity of the glycoprotein. NPG presenting covalently bound glycoproteins was used as the basis for a competitive electrochemical assay for glycoproteins in solution (transferrin and IgG). A kinetic ELLA based on steric hindrance of the enzyme-substrate reaction and hence reduced enzymatic reaction rate after glycoprotein binding is demonstrated using immobilized Con A-ALP conjugates. Using the immobilized Con A-ALP conjugate, the binding affinity of immunoglobulin G (IgG) was found to be 105 nM, and that for transferrin was found to be 650 nM. Minimal interference was observed in the presence of 5 mg mL(-1) BSA as a model serum protein in both the kinetic and competitive ELLA. Inhibition studies were performed with methyl alpha-D-mannopyranoside for the binding of TSF and IgG to Con A-ALP; IC50 values were found to be 90 mu M and 286 mu M, respectively."
According to the news editors, the research concluded: "Surface coverages of proteins were estimated using solution depletion and the BCA protein concentration assay."
For more information on this research see: Square-wave voltammetry assays for glycoproteins on nanoporous gold. Journal of Electroanalytical Chemistry, 2014;717():47-60. Journal of Electroanalytical Chemistry can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also Proteins).
Our news journalists report that additional information may be obtained by contacting B. Pandey, University of Missouri, Center Nanosci, St Louis, MO 63121, United States. Additional authors for this research include J.K. Bhattarai, P. Pornsuriyasak, K. Fujikawa, R. Catania, A.V. Demchenko and K.J. Stine.
Keywords for this news article include: Missouri, St. Louis, Chemistry, Nanoporous, United States, Glycoproteins, Nanotechnology, Electrochemical, Glycoconjugates, Emerging Technologies, North and Central America
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