News Column

Researchers from University of Kurdistan Report on Findings in Gene Therapy

June 5, 2014

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Fresh data on Biotechnology are presented in a new report. According to news reporting originating in Sanandaj, Iran, by NewsRx journalists, research stated, "Human keratinocytes could be used in the repair of damaged skin, in tissue engineering applications, gene therapy and recently, the generation of iPS cells. We isolated human keratinocytes from foreskin and subsequently cultured them on fibronectin, collagen type I, gelatin and laminin-coated dishes that contained three different types of serum-free medium (epilife, KSM or CnT)."

The news reporters obtained a quote from the research from the University of Kurdistan, "We developed improved conditions for efficient transfection of these human keratinocytes by testing three common transfection methods and a GFP plasmid vector. The isolated cells showed typical keratinocyte morphology and expressed the epithelial cell specific antigen, cytokeratin 14. Collagen type 1, epilife medium and lipofectamin 2000 gave the best results for isolation and transfection of human keratinocytes."

According to the news reporters, the research concluded: "Our protocol can be used as a reproducible, simple and efficient method for isolation, cultivation and genetic manipulation of human keratinocytes, which may be useful in cell and gene therapy applications."

For more information on this research see: Isolation, cultivation and transfection of human keratinocytes. Cell Biology International, 2014;38(4):444-51. (Elsevier -; Cell Biology International -

Our news correspondents report that additional information may be obtained by contacting S. Zare, Dept. of Biological Sciences and Biotechnology, Faculty of Science, University of Kurdistan, Sanandaj, Iran. Additional authors for this research include M.A. Zarei, T. Ghadimi, F. Fathi, A. Jalili and M.S Hakhamaneshi (see also Biotechnology).

Keywords for this news article include: Iran, Asia, Biotechnology, Tissue Engineering, Biomedical Engineering, Biomedicine, Sanandaj, Gene Therapy, Keratinocytes, Bioengineering.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC

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Source: Gene Therapy Weekly

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