By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Biotechnology have been published. According to news originating from Ames, Iowa, by NewsRx correspondents, research stated, "The effective treatment of central nervous system diseases is a major challenge due to the presence of the blood-brain barrier (BBB). P-aminophenyl-alpha-D-mannopyranoside (MAN), a kind of mannose analog, was conjugated onto the surface of liposomes (MAN-LIP) to enhance the brain delivery."
Our news journalists obtained a quote from the research from Iowa State University, "In this study, we investigated the brain distribution of MAN-LIP based on our previous studies and tried to explore the relationship between the distribution ofMAN-LIP and glucose transporters (GLUTs) on the cells. In vivo optical imaging was used to assess the distribution of liposomes in mice brain. The mice administered with MAN-LIP had significantly higher brain fluorescence intensity and MAN-LIP relatively concentrated in the cerebellum and cerebral cortex. Fluorescent microscope and Western blot were used to evaluate the results of lentiviral vector-mediated hSLC2A1 and hSLC2A3 gene transfection into C6, PC12 and vessels of endothelial cell line, bEND. 3. The results from live cell station and flow cytometry showed that the cellular uptake of MAN-LIP was significantly improved by GLUT1 and GLUT3 overexpression cells. The transport experiments also demonstrated that the transendothelial ability of MAN-LIP was much stronger when crossing LV-GLUT1/bEND. 3 cell monolayers or LV-GLUT3/bEND. 3 cell monolayers, of which GLUT1 and GLUT3 were overexpressed."
According to the news editors, the research concluded: "The combined data indicated that the transcytosis by GLUT1 and GLUT3 was a pathway of MAN-LIP into brain, and the special brain distribution of MAN-LIP was closely related to the non-homogeneous distribution of GLUT1 and GLUT3 in the brain. ."
For more information on this research see: The role of glucose transporters in the distribution of p-aminophenyl-alpha-D-mannopyranoside modified liposomes within mice brain. Journal of Controlled Release, 2014;182():99-110. Journal of Controlled Release can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Controlled Release - www.elsevier.com/wps/product/cws_home/502690)
The news correspondents report that additional information may be obtained from D. Du, Iowa State University, Dept. of Civil Construct & Environm Engn, Ames, IA 50011, United States. Additional authors for this research include N.D. Chang, S.L. Sun, M.H. Li, H. Yu, M.F. Liu, X.Y. Liu, G.T. Wang, H.C. Li, X.P. Liu, S.L. Geng, Q. Wang and H.S. Peng (see also Biotechnology).
Keywords for this news article include: Ames, Iowa, Biotechnology, Liposomes, United States, North and Central America
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